摘要
目的探讨间歇性负压对兔前交叉韧带重建后腱-骨界面及关节液中基质金属蛋白酶-9（MMP）-9及转化生长因子-β（TGF）-β表达的影响。方法新西兰大白兔24只，随机取一侧膝作为负压侧，对侧膝作为对照。取兔自体半腱肌作为重建材料，负压侧关节通过引流管接微型负压吸引器，维持间歇性、低强度负压，对照侧接常规引流管；5 d后两侧同时拔除吸引管。术后6周；取关节液和股骨-韧带-胫骨复合体，检测关节液和腱-骨界面MMP-9及TGF-β的表达。结果1只兔关节感染，共23只兔纳入研究。关节液检测提示负压组关节滑液中MMP-9含量低于对照组[（8.9±1.3） pg/L比（12.7±1.8） pg/L（P＝0.002）]；同时TGF-β含量高于对照组[（19.0±2.2） pg/L比（15.2±1.4） pg/L （P＝0.000）]。免疫组织化学研究发现，负压组腱-骨界面中MMP-9的表达低于对照组（P＝0.000）；而TGF-β的表达高于对照组，且差异有显著性（P＝0.000）。结论间歇性负压可以下调MMP-9及上调TGF-β在关节液和腱-骨界面的表达，促进了腱-骨的愈合。
ObjectiveTo study the effect of intermittent negative pressure on matrix metalloproteinase 9 （MMP）-9 and transforming growth factor β of tendon-bone interface and joint fluid after reconstruction of anterior cruciate ligament in rabbits.MethodsA total of twenty-four New Zealand white rabbits were randomly selected hind leg of negative group, contralateral hind leg as control.Reconstruction of the anterior cruciate ligament was done by autogenous semitendinosus of rabbit.Joint of the negative pressure side placed drainage tube connecting the micro-negative pressure aspirator, and maintained an intermittent, low-intensity negative pressure.Control side placed ordinary drainage tube.Drainage tube of both sides was pulled out at the same time after 5 days.After 6 weeks, joint fluid and femur-ligament-tibia complex were obtained for study of expression of MMP-9 and TGF-β in joint fluid and tendon-bone interface.ResultTwenty-three rabbits were included in the study because of one rabbit joint infections.Detection of joint fluid showed that MMP-9 content is significantly lower in negative group than that in the control group, and the difference is statistically significant [（8.9±1.3） pg/L vs （12.3±1.8） pg/L （P=0.002）]. TGF-β content is significantly higher in negative group in joint fluid than that in the control group, and the difference is statistically significant [（19.0±2.2） pg/L vs （15.2±1.4） pg/L （P=0.000）]. Study of immunohistochemistry in tendon-bone interface found that expression of MMP-9 is lower in negative pressure group than that in the control group, and the difference is statistically significant （P=0.000）. TGF-β expression is significantly higher in negative group in tendon-bone interface than that in the control group, and the difference is statistically significant （P=0.000）.ConclusionIntermittent negative pressure may reduce content of MMP-9 in joint fluid and expression of MMP-9 in tendon-bone interface, increase content of TGF-β in joint fluid and expres
出处
《中华医学杂志》
CAS
CSCD
北大核心
2017年第45期3583-3587,共5页
National Medical Journal of China
基金
陕西省自然科学基础研究计划项目（2011JM4050）
关键词
前交叉韧带
负压
腱-骨愈合
基质金属蛋白酶-9
转化生长因子-β
Anterior cruciate ligament
Negative pressure
Tendon-bone healing
Matrixmetalloproteinase 9
transforming growth factor β
