摘要
[目的]诊断疑似患有克雷伯氏杆菌病的发病鸡群。[方法]通过采取病鸡肝脏和脑组织,接种于胰蛋白胨琼脂培养基、麦康凯琼脂培养基、SS琼脂培养基、兔血琼脂培养基,培养出疑似克雷伯氏杆菌菌落,革兰氏染色发现该菌属于革兰氏阴性菌,将菌落进行溶血试验和生化试验。[结果]初步判断该菌为克雷伯氏杆菌。药敏试验中发现该菌对庆大霉素、妥布霉素、恩诺沙星、阿米卡星表现较敏感;对阿莫西林、卡那霉素、氨苄西林、头孢噻肟、头孢曲松、多黏菌素、四环素有极强的耐药性;对于红霉素、新霉素的敏感性介于两者之间。[结论]药敏试验为治疗该菌提供了大致方向,同时提醒抗生素类药物切不可滥用,以免产生耐药性。
[ Objective ] To diagnose the chickens suspected of having Klebsiella brucellosis. [ Method] By using the liver and brain tissue of the diseased chicken, we inoculated in tryptone agar medium, MacConkeyagar medium, SS agar medium and rabbit blood agar medium to cul-ture the suspected Klebsiella brucellosis colony. Gram stain found that the strain belonged to Gram-negative bacteria, the colony was subjected to hemolysis and biochemical tests. [ Result] The results preliminarily reflected the suspected bacteria was Klebsiella brucellosis. The drug sen-sitivity test showed that the bacteria had strong drug resistance to amoxicillin, kanamycin, ampicillin, cefotaxime, ceftriaxone, polymyxin and tetracycline, more sensitivity to gentamicin, tobramycin, enrofloxacin, amikacin,and erythromycin, neomycin between them. [ Conclusion] Drug sensitive test provides direction for treatment of the bacteria, but also reminds drugs must not be abused in order to avoid drug resistance.
出处
《安徽农业科学》
CAS
2017年第34期92-94,共3页
Journal of Anhui Agricultural Sciences
基金
安徽省农业科学院学科建设项目(16A0411)
国家现代农业产业技术体系项目(CARS-40)
关键词
克雷伯氏杆菌
生化试验
药敏
Klebsiella
Biochemical tests
Drug sensitivity
