摘要
目的 研究核糖体蛋白L5（ribosomal protein L5,RPL5）在儿童和成人甲状腺乳头状癌中的差异表达及其诊断价值.方法 采用实时荧光定量PCR方法检测甲状腺乳头状癌（papillary thyroid carcinoma,PTC）癌组织和癌旁正常组织中RPL5表达情况,包括癌组织样本22例和癌旁组织样本13例（儿童甲状腺乳头状癌样本10例,癌旁组织3例;成人甲状腺乳头状癌标本12例,癌旁组织10例）.同时采用受试者工作特征曲线（receiver operating characteristic curve,ROC）中两个重要指标：曲线下面积（area under the curve,AUC）和Youden指数（Youden′s index）对RPL5在甲状腺乳头状癌中的诊断价值进行评估.结果 与癌旁正常组织相比,PTC肿瘤组织中RPL5表达显著增高（P=0.001）,同时成人PTC肿瘤组织中RPL5表达显著高于儿童PTC（P=0.044）.RPL5诊断成人和儿童PTC的AUC=0.820（P=0.001）,Youden指数=0.568;儿童PTC对应的AUC=0.721（P=0.069）,Youden指数=0.414,而在成人分别为AUC=0.896（P=0.0005）,Youden指数=0.709）,明显高于儿童PTC.进一步分析发现,RPL5在成人PTC的AUC（0.896,Youden指数0.709）要好于目前临床常用指标CK19（AUC=0.857,Yoden指数0.774）、Galectin-3（AUC 0.868,Youden指数0.775）和甲状腺过氧化物酶（AUC 0.830,Youden指数0.698）.结论 本研究阐明RPL5在PTC肿瘤中表达显著高于癌旁组织,并明确RPL5对PTC具诊断价值.此外,本研究还提示RPL5作为分子标志物在成人PTC中的诊断价值明显高于儿童PTC,有助于提高PTC临床诊断.
Objective To study the difference expression and diagnostic value of ribosomal protein L5 （RPL5） in papillary thyroid carcinoma （PTC） of children and adults.Methods Realtime-PCR was performed to detect the expression of RPL5 in 22 PTC tissues and 13 pericarcinous tissues.Receiver operating characteristic （ ROC） curve and Youden＇s index were used to evaluate the diagnostic value of RPL 5＆nbsp;in PTC of children and adults.Results The expression of RPL5 in PTC tissues was higher than in pericarcinous tissues.The area under curve （AUC） was 0.820 （P =0.001）, and Youden′s index was 0.568.The expression of RPL5 in PTC of adults was higher than children （ P 〈0.05 ） .The AUC and Youden＇s index were respectively 0.721 （P=0.069） and 0.414 in children, whereas being respectively 0.896 （P=0.0005） and 0.709 in adults.RPL5 in diagnosis of PTC of adults was better than CK19, Galectin-3 and TPO, which are commonly used for the pathologic diagnosis of PTC .Conclusion The expression of RPL5 in PTC is higher than pericarcinous tissues , and its expression in PTC of adults is higher than children.Furthermore, PTC is a potential indicator for diagnosis of PTC .
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2017年第11期830-834,共5页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
国家自然科学基金（81502144）
北京市科技计划（D131100005313014）
北京市医院管理局重点医学专业发展计划资助（ZYLX201508）
关键词
核糖体蛋白质类
甲状腺肿瘤
癌
乳头状
诊断价值
受试者工作特征曲线
Ribosomal proteins
Thyroid neoplasms
Carcinoma, papillary
Diagnostic value
Receiver operating characteristic curve
