摘要
针对厌氧反硝化工艺在处置高浓度硝酸盐废液过程中难以连续运行的实际情况,系统研究了升流式污泥床(USB)反应器恢复启动过程中微生物群落变化过程。结果表明:采用原水NO-3-N负荷的30%(710.7 mg·L^(-1))恢复启动23 d,NO-3-N平均去除率为99%,比反硝化速率(RNO-3)从49.4 mg·(g·h)-1提升到170.7 mg·(g·h)-1(以VSS计),提高了3~4倍。高通量测序分析发现,Halomonas(盐单胞菌属)、Pseudomonas(假单胞菌属)、Alcaligenes(产碱杆菌属)为脱氮的优势菌种,与启动初期相比,启动末期的相对丰度分别增长了35%、31%和14%。优势菌种的相对丰度与RNO-3之间存在一定的正相关性,RNO-3每增加1倍,优势菌种的相对丰度平均扩增6.7%。USB厌氧反硝化反应器可以快速恢复启动,具有较好的工程应用可行性和运行灵活性。
Anaerobic denitrification is feasible for the disposal of waste liquids with extremely high nitrate （NO3--N） concentration. This study systematically investigates the microbial community variation during the re- startup of the up-flow sludge bed （USB） reactor. Results indicated that the average NO3- -N removal was 99% during the 23 d re-startup period with influent NO3-N concentration of 710.7 mg· L-1. The specific denitrifica- tionrate（RNO3-）increased from 49.4 mg · （g · h） -1to 170.7 mg - （g·h） （based on VSS）. The high- throughput DNA sequencing technology showed that Halomonas, Pseudomonas, and Alcaligenes were the domi- nant species of denitrifying bacteria, and their relative abundance after re-startup were increased by 35% , 31% and 14% , respectively. The relative abundance was positively correlated with the specific denitrification rate. The double value of Rno3- was relative to 6.7% increase of the abundance of the dominant species. USB anaero- bic denitrification reactor could be re-startup quickly and was potentially available for denitrification with good feasibility and operational flexibility.
出处
《环境工程学报》
CAS
CSCD
北大核心
2017年第11期5917-5922,共6页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金优秀青年基金资助项目(51422813)
关键词
高浓度硝酸盐废液
厌氧反硝化
比反硝化速率
微生物群落特征
反应器恢复启动
high-nitrate waste liquid
anaerobic denitrification
specific denitrification rate
microbial communitycharacteristics
reactors re-startup
