摘要
目的:探讨大鼠在亚高原及高原环境下,经过力竭运动后不同时间内氧化损伤及炎症反应的变化。方法:72只大鼠随机分为亚高原组和高原组,每组36只,按照取样时间不同再分别将亚高原组和高原组随机分为6个亚组:相同海拔高度对照组,力竭后0、6、12、24、48h组,每组6只。通过使大鼠进行负重游泳至力竭,记录游泳时间,检测力竭后不同时间血清中丙二醛(MDA)、超氧化物歧化酶(T-SOD)以及肿瘤坏死因子-α(TNF-α)的含量。结果:高原组力竭游泳时间明显短于亚高原组。在亚高原环境下,大鼠力竭运动后血清中MDA及TNF-α无显著变化;与亚高原对照组相比,T-SOD含量在力竭后12h显著下降(P<0.01),24h时又恢复到对照组水平。在高原环境下,MDA含量在力竭运动后6h显著升高(P<0.01),12h、24h时持续升高(P<0.01),48h时有所下降,但仍高于高原对照组(P<0.05);T-SOD含量在力竭后6h时显著降低(P<0.01),12h、24h时有所升高,但仍低于高原对照组(P<0.05),48h时达到对照组水平;血清中TNF-α的含量在24h时显著升高,48h时仍维持在较高水平(P<0.01)。结论:大鼠在亚高原环境下,力竭运动后产生的氧化损伤及炎症反应较小,而在高原环境下,大鼠体能下降,经过力竭运动后,机体的氧化损伤以及炎症反应大幅度增强。
Objective： To investigate the changes of oxidative damage and inflammatory reaction at different time in rats after subjected exhaustive exercise in plateau and sub--plateau environment.Methods：A total of 72 rats were randomly divided into high altitude group （n=6） and sub--high altitude group （n = 36）.Then each group of high altitude and sub--high altitude were randomly divided into six groups （n = 6） according to sampling time. The rats were carried out weight--bearing swimming test till exhaustion. The swimming time was recorded, and MDA, T-- SOD and TNF-a were determined.ResuLts： The swimming time ofhigh altitude group was significantly shorter than that of sub--high altitude group.The content of serum MDA and TNF-a had no obvious changes in sub--high altitude group. Compared with the control group at sub--high altitude, the content of serum T--SOD were significantly deceased at 12 h （P〈0.01） and then increased to control level at 24 h.In the high altitude,the content of serum MDA significantly increased at 6 h（P〈0.01） and was still on the rise at 12 h and 24 h,as time continues to 48 h,there was a decline hut still higher than the control group （P〈0.05）.The content of T--SOD was significantly deceased at 6 h （P〈0.01） ,when the time continues to 12 h and 24 h,it was increased but still lower than the control group（P〈0.05）, then returned to control level at 48 h.The content of TNF--a was significantly inceased at 24 h （P 〈0.01） and remained high at 48 h （P 〈 0.01 ）. Conclusion. Compared with the sub -- high altitude group,rats in the high altitude group have a reduction of physical ability. There were much more oxidative damage and inflammatory reaction after exhaustive exercise.
出处
《西北国防医学杂志》
CAS
2017年第10期631-634,共4页
Medical Journal of National Defending Forces in Northwest China
基金
全军医药卫生科研基金面上项目(CLZ12JA08)
关键词
高原
力竭运动
氧化损伤
炎症反应
high altitude, exhaustive exercise, oxidative damage, inflammatoryreaction
