摘要
目的:揭示运动性疲劳发生、发展过程中纹状体脑区抗坏血酸(AA)动态变化规律及其对胞外Glu浓度的调节作用,进一步阐明AA抗运动性疲劳的中枢机制。方法:以雄性Wistar大鼠为实验对象,实验分为AA检测组(AAT)、AA补充组(AAG)和对照组(SG),采用微透析—电化学联用检测技术,动态观察一次性力竭运动过程中大鼠纹状体细胞外AA浓度的变化;采用微透析—高效液相色谱联用技术,动态观察外源性补充AA对纹状体胞外Glu含量的影响。结果:AAT大鼠纹状体胞外AA浓度在运动初期未见著改变,运动后期与安静状态相比显著升高(P<0.01),恢复90 min时仍显著高于安静水平(P<0.05)。补充AA后,力竭性运动过程中大鼠纹状体胞外Glu与对照组的变化趋势相似,但Glu峰值出现的时间较对照组推迟约30 min,且大鼠运动至力竭的时间也显著延长(P<0.05)。结论:大鼠在力竭运动过程中纹状体胞外AA浓度升高可能是对自身抗氧化能力降低的一种代偿反应;运动前适当补充AA可以降低纹状体Glu浓度异常升高所产生的兴奋性毒性作用,延缓运动疲劳的发生,其机制可能与AA的抗氧化和对纹状体胞外Glu的调节作用有关。
Objective：The purpose of this study is to reveal the dynamic changes of ascorbic acid （AA） in the striatum and the regulation of extracellular Ghi concentration during the development of exercise-induced fatigue, and to further clarify the central mechanism of AA anti exercise fatigue. Male Wistar rats were used as experimental subjects, and divided into AA group （AAT） ,AA supplement group （AAG） and control group （SG）. The changes of extracellular AA concentration in striatum of rats in the process of one-time exhaustive exercise were observed dynamically by micro dialysis-electrochemical detection technology. The effects of exogenous AA supplementation on the content of extracellular Glu in striatum were observed by micro-dialysis and high performance liquid chromatography （HPLC）. Results：In the AAT group, the extracellular AA concentration in the striatum did not change in the early stage of exercise, the late stage of exercise was significantly higher than that of quiet state （P 〈 0.01 ）, and the recovery of 90min was still significantly higher than that of the quiet level （P 〈 0. 05 ）. After supplementing AA, the trend of Glu in the striatum of rats during exhaustive exercise was similar to that in the control group, but the peak value of Glu was delayed by about 30rain compared with the control group, and the time of exercise to exhaustion was significantly prolonged （ P 〈 0.05 ）. Conclusion ： The increase of extracel- lular AA concentration in the striatum during exhaustive exercise and recovery may be a compensatory response to the decrease of antioxidant capacity. Supplement AA can decrease the excitatory toxic effect caused by the abnormal increase of Glu concentration in the striatum and delay the occurrence of exercise fatigue, which may be related to the antioxidation of AA and the regulation of extracellular Glu in the striatum.
出处
《沈阳体育学院学报》
CSSCI
北大核心
2017年第5期70-75,共6页
Journal of Shenyang Sport University
基金
国家自然科学基金资助项目(31571221)
北京自然科学基金资助项目(5142012)
