摘要
目的:探讨运动性中枢疲劳时大鼠大脑皮质MCT1、MCT2mRNA表达的变化以及乳酸对大鼠大脑皮质神经元MCT2mRNA表达的影响。方法:12只Wistar大鼠随机分为对照组和力竭训练1周组,每组6只。通过7天力竭训练建立中枢疲劳模型,观察中枢疲劳时大鼠大脑皮质MCT1、MCT2mR-NA表达变化;采用原代培养的大鼠大脑皮质神经元,观察不同浓度乳酸作用后大鼠大脑皮质神经元MCT2mRNA表达的变化。采用Trizol法提取大脑皮质和神经元总RNA;用SYBRAgreenI荧光定量RT-PCR测定MCT1及MCT2mRNA表达变化。结果:整体水平:力竭训练1周组MCT1mRNA表达无明显变化,而MCT2mRNA表达较对照组显著升高6·35±3·54倍(P<0·05)。细胞水平:乳酸作用后,神经元MCT2mRNA表达在pH=7·25时较对照组(pH=7·35组)升高3·08±2·38倍(P<0·05)。但随着乳酸浓度进一步升高,MCT2mRNA表达无明显增加。结果提示,中枢疲劳可能引发大脑皮质神经元MCT2mRNA表达升高,MCT2基因表达可能与乳酸在中枢疲劳中的作用有关。
The present study was undertaken to explore the changes of monocarboxylate transporter （MCT） MCT1 and MCT2 mRNA expression in rat cortex during central fatigue induced by exercise and to detect the effects of lactic acid on cortex neuronal MCT2 mRNA expression. Central fatigue model was built through 7 - day exhaustive training. At the last day of training, the rats were decollated for extracting total RNA from the cortex. Cortical neurons of rats were kept in primary cultures. Trizol was used to extract cortex and cortical neuronal total RNA; while the changes of MCT1, MCT2 mRNA expression were detected by SYBRA green I one step RT- PCR. The results showed that the expression of MCT2 mRNA was obviously increased in the central fatigue group （6.35 ± 3.54 folds increase） （ P 〈 0.05）. After adding lactic acid, slight further increase in neuronal MCT2 mRNA expressions was seen, especially significant alteration in the group of pH = 7.25 （3.08 ± 2.38 folds increase）（ P 〈 0.05）, whereas MCT1 mRNA changed insignificantly. The results suggested that the expression of MCT2 mRNA was up regulated by exogenously added lactic acid, which could be relevant to the possible effects of lactic acid in the occurrence of central fatigue.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2007年第1期52-55,62,共5页
Chinese Journal of Sports Medicine
