摘要
目的探讨强直性脊柱炎(AS)患者血清及关节液中S100A8/9水平变化的临床意义。方法选择门诊和住院的AS患者55例,以常用AS疾病活动指数(BASDAI)中位数3.1为界分为活动期组19例和非活动期组36例,选择同期健康体检人员20例作为正常对照组。采用ELISA双抗体夹心法测定S100A8/9水平,采用魏氏法测定ESR,采用Array-360特定蛋白分析仪测定C反应蛋白(CRP)。比较各组血清S100A8/9、CRP和ESR水平,同时比较活动期组患者治疗前后血清S100A8/9水平,并分析其与AS活动参数的相关性。结果 55例患者血清S100A8/9水平高于正常对照组,差异有统计学意义(P<0.01)。15例AS患者关节液中S100A8/9水平高于其血清水平,差异有统计学意义(P<0.01)。活动期组患者血清S100A8/9、CRP和ESR水平均高于非活动期组患者,差异均有统计学意义(均P<0.01)。19例活动期组患者治疗3个月后平均BASDAI值和血清S100A8/9水平均较治疗前下降,差异均有统计学意义(均P<0.01)。55例AS患者血清S100A8/9水平与BASDAI呈正相关(r=0.336,P<0.05),而与ESR、CRP均无相关性(r=0.051和0.067,均P>0.05)。结论 AS患者外周血清S100A8/9水平与疾病活动度密切相关,检测AS患者血清S100A8/9水平对评价疾病活动度和疗效判断有一定价值。
Objective To investigate the clinical significance of S100 A8/9 levels in serum and synovial fluid of patients with ankylosing spondylitis（AS）. Methods Fifty five patients with AS were included in the study, including 19 cases of active AS and 36 cases of non-active AS according to Bath Ankylosing Spondylitis Disease Activity Index（BASDAI） median（3.1）, and 20 healthy subjects served as the control group. The levels of S100 A8/9 in serum and synovial fluid were measured by ELISA assay before and after the treatment. The level of S100 A8/9, CRP and ESR were compared in the three groups. The correlation between S100 A8/9 and BASDAI was analyzed. Results Serum S100 A8/9 levels in 55 patients with AS were significantly higher than those in controls（P〈0.01）. The S100 A8/9 levels in synovial fluid from 15 AS patients were significantly higher than those in serum（P〈0.01）. The serum levels of S100 A8/9, CRP and ESR in active AS patients were significantly higher than those in non-active AS patients（all P〈0.01）. After three months of treatment, the serum levels of S100 A8/9 and BASDAI from 19 patients with active diseases were significantly decreased. Serum levels of S100 A8/9 in 55 AS patients were positively correlated with BASDAI（r=0.336, P〈0.05）, but not correlated with CRP and ESR levels（（r=0.051 and 0.067, both P〈0.05）. Conclusion Serum S100 A8/9 levels are associated with active disease in AS patients, indicating that serum S100 A8/9 levels might be a potential marker for disease activity in AS.
出处
《浙江医学》
CAS
2017年第21期1895-1897,1900,共4页
Zhejiang Medical Journal
基金
湖州市科学技术局项目(2016GYB24)
