摘要
目的:研究白藜芦醇(Resveratrol,Res)对荷子宫内膜癌裸小鼠移植瘤生长的抑制作用并探讨其作用机制。方法:采用皮下注射接种子宫内膜癌Ishikawa细胞的方法建立荷子宫内膜癌裸小鼠模型,给予不同剂量Res[50、100、200 mg/(kg·d)]进行干预治疗,疗程5天。观察移植瘤生长情况,称取瘤重并计算抑瘤率,苏木精-伊红(HE)染色法观察肿瘤组织形态结构改变;末端标记法(TUNEL)观察肿瘤组织细胞凋亡状况并计算凋亡指数(apoptosis index,AI);蛋白免疫印迹(Western blot)法检测肿瘤组织中Bax、bcl-2、caspase-3蛋白表达并计算Bax/bcl-2比值。结果:与模型对照组比较,发现经Res治疗后荷子宫内膜癌裸小鼠移植瘤瘤体减小、活动度较差且硬度较差,以200 mg/(kg·d)组最为显著;Res[100、200 mg/(kg·d)]组瘤体重量显著降低(P<0.01),Res[200、100、50 mg/(kg·d)]组抑瘤率均显著升高(P<0.01);HE染色后光镜下可见经Res干预能够诱发荷子宫内膜癌裸小鼠移植瘤组织细胞皱缩、片状坏死等病变;TUNEL染色后光镜下可见经Res干预能够导致移植瘤组织凋亡细胞数量明显增多,显著提高AI(P<0.01);显著上调肿瘤组织Bax、caspase-3蛋白表达并下调bcl-2蛋白表达(P<0.01),提高Bax/bcl-2表达比值(P<0.01)。结论:Res对荷子宫内膜癌裸小鼠移植瘤生长具有一定的抑制作用,其机制可能与Res能够调节凋亡相关蛋白表达促进肿瘤细胞凋亡有关。
Objective：To investigate the effects and mechanism of Resveratrol （Res） on xenografts growth in en- dometrial cancer of nude miee. Methods：Nude mice models bearing human endometrial cancer were made by injec- ting inoculation human endometrial cancer Ishikawa cell line and were treated with different eoneentrations of Res [ 50,100,200 mg/（ kg·d）] for 5 d, once a day. The general states was observed, the tumor volum, tumor weight were detected and the inhibition rate was calculated. The histopathological changes of tumor tissue was observed by HE staining. The tumor cells apoptosis was detected by TUNEL. The expression of caspase - 3, Bax, bcl - 2 protein were detected by Western blot, and the ratio of Bax/bcl - 2 was calculated. Results： Compared with model control group,the rats in Res groups appeared significantly reduced the tumor, poor activity, the hardness difference, especial- ly the mice in the Res 200 rag/（ kg·d） group. The tumor weight in Res [ 100,200 rag/（ kg·d） ] groups were sig- nifieantly decreased and the inhibition rate were significantly increased （P 〈 0.01 ）. HE staining showed that the nec- rosis presentation, tumor cell shrinkage and other pathological morphological changes appeared. TUNEL staining showed that the number of apoptotic cells in tumor tissue of Res groups were significantly increased, the AI in Res [ 100,200 rag/（ kg·d）] groups were significantly decreased （P 〈 0.01 ）. The expression of Bax and caspase- 3 were significantly increased and the bcl - 2 protein was significantly decreased （ P 〈 0.01 ） , the ratio of Bax/bcl - 2 were significantly increased （P 〈 0.01 ）. Conclusion： Res has inhibitive effects on xenografts growth in endometrial cancer of nude mice,which perhaps related to its effects of promoting apoptosis by altering the expression of apoptosis - related proteins.
出处
《现代肿瘤医学》
CAS
2017年第23期3741-3745,共5页
Journal of Modern Oncology
基金
沧州市科技计划项目(编号:151302163)
关键词
白藜芦醇
子宫内膜癌
移植瘤
生长
凋亡
Resveratrol, endomelrial ~ancer, xenografls, growth, apoptosis
