摘要
目的:采用ITS2序列对博落回属药用植物进行鉴定。方法:通过对来自湖南、江西、浙江、湖北、河南、陕西、重庆的30个博落回属植物ITS2序列进行扩增和测序,并对其进行扩增效率及测序成功率、碱基构成、种内和种间变异分析及NJ系统进化树分析来评估ITS2序列鉴定效果。结果:博落回属药用植物ITS2序列长度在500 bp以内,扩增效率和测序成功率为100%,并获得30条博落回ITS2序列。结论:ITS2序列可以有效准确鉴别博落回属植物。
Objective： To identify the medicinal plant Macleaya spp. by using ITS2 sequence. Methods： The ITS2 sequence and sequence of 30 samples collected from Hunan, Jiangxi, Zhejiang, Hubei, Henan, Shanxi, Chongqing was amplified and the amplification efficiency, the sequencing success rate, the constitution of base pair, intra- and inter-specific variation and NJ（Neighbor-Joining） tree were analyzed, in order to evaluate the ITS2 sequencing results. Results： The ITS2 sequence length of Macleaya spp. was within 500 bp ; the amplification efficiency and the success sequencing rate was 100% and 30 ITS2 sequences were obtained. Conclusion： The ITS2 sequence can be effective and accurate for identification of Macleaya spp.
出处
《中国现代中药》
CAS
2017年第10期1367-1370,1381,共5页
Modern Chinese Medicine
基金
国家重点实验室培育基地开放项目(15KFXM09)
湖南省科技重点计划(2016SK3002)
