摘要
为了解华北地区猪繁殖与呼吸综合征病毒(PRRSV)的遗传变异情况,通过对2014—2016年从河南、河北、山东、山西4省采集的疑似猪繁殖与呼吸综合征(PRRS)病料进行检测,将扩增得到的PRRS阳性样品的ORF5基因进行测序,分析华北地区2014—2016年PRRSV的变异情况。结果表明,2014—2016年华北地区流行毒株与国内外代表性毒株核苷酸同源性在84.6%~99.8%;抗原位点比对结果显示,变异株中和表位第37—44位氨基酸为SH(I/L/F)QLIY(N/K),诱骗表位27—30位氨基酸为(V/A)L(V/A)N;系统进化树分析表明,以CH-1a为代表的变异株、以VR-2332为代表的经典毒株以及NADC30毒株在华北地区同时存在。可见,华北地区PRRSV流行情况复杂。
In order to elucidate the evolution of the porcine reproductive and respiratory virus（ PRRSV） in northern China from 2014 to 2016,suspicious samples collected from Henan,Hebei,Shandong and Shanxi province were subject to PRRSV diagnosis,the positive samples were further subject to RT-PCR to obtain the sequence of ORF5. The sequence acquired were further aligned with the ORF5 sequence available in Gen Bank to construct phylogenetic tree. Also,antigenic sites of ORF5 compared to those of other isolates to analyse whether the antigenic sites has changed or not. Sequence comparision showed that the homology of ORF5 gene obtained in the present study was up to 84. 6% —99. 8%. Antigenic site comparison showed that neutralizing epitipes of the HP-PRRSV located at 37—44 were SH（ I/L/F） QLIY（ N/K）,decoy epitope located at 27—30 were（ V/A） L（ V/A） N. Phylogenetic tree showed that HP-PRRSV with the representive strain CH-1 a,together with classic PRRSV represented by VR-2332 and NADC30 strain have been circulating in northern China. In conclusion,the PRRSV has been increasingly complicated.
出处
《河南农业科学》
CSCD
北大核心
2017年第10期128-131,共4页
Journal of Henan Agricultural Sciences
基金
河南省科技攻关项目(162102110050
162102110042)
河南省农业科学院自主创新基金项目(2016ZC51
2017ZC49)
关键词
猪繁殖与呼吸综合征病毒
ORF5
序列分析
进化
porcine reproductive and respiratory syndromevirus（ PRRSV ）
ORF5
sequence analysis
evolution
