摘要
目的:观察力竭运动应激后小鼠脾脏T、B淋巴细胞钾离子通道表达的差异性,探讨力竭运动应激对T、B淋巴细胞的影响。方法:复制小鼠力竭模型后从脾脏中分离出T、B淋巴细胞,测定淋巴细胞转化功能,溶血空斑形成实验,流式细胞仪T淋巴细胞周期和分型,RT-PCR、Western blot测小鼠脾脏T、B细胞Kv1.3的表达。结果:小鼠脾脏T淋巴细胞Kv1.3 mRNA和蛋白质表达水平明显下调(P<0.05),小鼠脾脏B淋巴细胞Kv1.3 mRNA和蛋白质表达水平没有显著性差异(P>0.05);力竭运动应激小鼠T淋巴细胞功能受抑制(P<0.05),B淋巴细胞功能未受到明显影响(P>0.05)。结论:力竭运动应激导致小鼠脾脏T、B淋巴细胞Kv1.3 mRNA和蛋白质表达呈现差异性,进而影响淋巴细胞功能,提示力竭运动应激条件下细胞免疫功能的抑制可能与钾通道有关,为进一步研究运动应激导致免疫抑制的机制提供了新的思路。
Purpose： This study aimed at observing different expressions of Kv1.3 of T and B lymphocytes in spleen of mice after exhaustive exercise,and exploring the effect of exhaustive exercise on T and B lymphocytes.Methods： T and B lymphocytes in spleen of mice were separated after exhaustive exercise; lymphocyte transformation test（ LTT）,plague forming cell（ PFC） test were performed to demonstrate their functions; RT-PCR,Western blot were performed to demonstrate the mRNA and protein expressions of Kv1.3 of T and B lymphocytes in spleen.Results： Compared with control group,the mRNA and protein expressions of Kv1.3 were significantly down-regulated in T lymphocyte of spleen after exhaustive exercise（ P 0.05）,and there were no significant differences of B lymphocyte after exhaustive exercise（ P 0.05）; transformation function of T lymphocyte was affected by exhausted exercise（ P 0.05） and that of B lymphocyte was not（ P 0.05）.Conclusion： Exhaustive exercise changes the mRNA and protein expressions of Kv1.3 of T and B lymphocytes in spleen of mice,affects the function of lymphocytes,which indicate that the inhibition of cellular immune function under exhaustive exercise stress may be related to potassium channels.This study provided a new idea for further research on the mechanism of exercise inducing immunosuppression.
出处
《北京体育大学学报》
CSSCI
北大核心
2017年第9期42-47,共6页
Journal of Beijing Sport University
基金
天津市创新团队资助项目
