摘要
拟南芥诱导型启动子rd29A作为低温及盐胁迫的诱导型启动子,其在长春花中的生物学功能为长春花转基因植物的构建提供了一个可控的基因表达体系。本研究通过克隆拟南芥启动子rd29A,替换双元载体p BI121中组成型启动子Ca MV 35S构建表达载体rd29A::GUS,经根瘤农杆菌(GV3101)介导转化长春花顶端分生组织实现GUS基因在rd29A启动子驱动下的瞬时表达。表明:同常温条件(25℃)相比,经低温诱导(4℃)12 h后长春花中GUS基因表达量升高了四倍,GUS染色也同时证明了低温可成功诱导rd29A启动子在长春花中的生物学活性,进而为长春花诱导型基因表达的转基因植物构建提供了可行的理论依据。
Arabidopsis rd29 A promoter could be induced by low-temperature and salt-stress and its biological activity for driving the expression of target genes in Catharanthus roseus makes it possible used for constructing transgenic plants. In this research, we cloned rd29 A promoter and replaced bianry vector p BI121 to Ca MV 35 S promoter to produce rd29A：：GUS expression vector, and achieved transient expression of GUS gene driven by rd29 A promoter by Agrobacterium tumefaciens mediated transformation of apical meristem in Catharanthus roseus（GV3101）. The transformed leaves showed that comparing with the experiment at room temperature（25℃）, GUS expression was four times higher inducing at low-temperature（4℃） for 12 hours. In the meanwhile indicated that low temperature could induce Arabidopsis promoter biological activity in Catharanthus roseus and also provided a theoretical basis for the construction of transgenic plants with induced gene expression in Catharanthus roseus.
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第9期3518-3523,共6页
Molecular Plant Breeding
基金
辽宁省自然科学基金项目(2015020682)资助
