摘要
本研究利用PCR技术从三倍体毛白杨((Populus tomentosa×P.bolleana)×P.tomentosa)基因组DNA中扩增获得抗病相关PtDRG02基因的一个启动子区(包括其下游5'端非编码区序列),命名为Ptp01。以β-葡萄糖苷酸酶(gus)基因为报告基因,构建了PtDRG02基因启动子植物表达载体Ptp01/gus。以根癌农杆菌EHA105及LBA4404感受态细胞为受体,转化植物表达载体Ptp01/gus进行瞬时表达,并对启动子进行了功能活性分析,结果表明该启动子能够驱动gus报告基因在毛白杨叶片组织中表达,但表达强度弱于花椰菜花叶病毒(CaMV)35S启动子。
A promoter fragment followed by 5＇ untranslated region （5＇UTR） of a disease resistance-related gene, PtDRG02, was PCR-amplified from the genomic DNA of triploid white poplar [（Populus tomentosaxP, bolleana）x P. tomentosa]. This fragement designated as Ptp01 was then translationally fused to the β-glucuronidase （gus） reporter gene, generating a plant expression vector Ptp01/gus. The obtained Ptp01/gus vector was subsequently transferred into Agrobacterium EHA105 and LBA4404 respectively. The Agrobacterium-mediated transient expression result compellingly indicated that the present PtDRG02 gene promoter was able to drive the gus reporter gene expression in the poplar leaf tissues, but the strength was weaker than that of the cauliflower mosaic virus （CaMV） 35 S promoter.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2009年第4期668-672,共5页
Genomics and Applied Biology
基金
国家自然科学基金项目(30872043)
教育部博士点基金项(20070022003)共同资助
