摘要
为优化白菜游离小孢子培养高频胚诱导技术体系,以20个不同基因型白菜栽培品种为供试材料,对影响白菜游离小孢子胚诱导和植株再生因素进行研究。结果表明:有14个基因型产生了小孢子胚,不同基因型之间胚诱导率差异显著,表明基因型是影响小孢子胚发生的主要因素;通过对白菜游离小孢子培养高频胚诱导技术体系的优化,胚诱导率明显提高,最优组合为盛花期取蕾;花瓣长与花药长比为0.5~0.75;4℃低温预处理1 d;150 g/L蔗糖的NLN培养基33℃高温热激2 d,重新离心更换130 g/L蔗糖的NLN培养;激素最优组合为6-BA0.1 mg/L、NAA 0.5 mg/L;在培养基中添加活性炭有利于胚的形成,最佳浓度为0.5 g/L。
To optimize the system of high frequency embryo induction for free microspore culture in Chinese cabbage, a total of 20 different genotypes were used as the experimental materials to study the factors that affecting the embryo induction of free microspore in Chinese cabbage and plantlet regeneration. The results showed that a total of 14 genotypes produced microspore embryo, significant differences in rate of embryo induction were observed among these genotypes, indicating that genotype was the main factor affecting the formation of microspore embryo. The rate of free microspore embryo induction was significantly improved by the optimization of high frequency embryo induction system for free microspore culture in Chinese cabbage. The optimal combination was the following： bud collected in the period of flowering, a ratio of petal length and anther length ranged from 0.5 to 0.75, bud in 4 ℃ condition for 1 d, then the bud in NLN culture medium containing 150 g/L sucrose and a heat shock conducted under 33 ℃ for 2 d. Finally, the bud was transferred into the culture medium containing130 g/L sucrose after centrifugation. The medium containing 0.1 mg/L 6-BA and 0.5 mg/L NAA could promote the microspore embryogenesis. It is beneficial for the formation of embryos by adding 0.5 g/L activated carbon.
出处
《热带作物学报》
CSCD
北大核心
2017年第10期1913-1920,共8页
Chinese Journal of Tropical Crops
基金
贵州省科技计划项目(黔科合重大专项[2013]6007号)
黔农科院自主创新科研专项([2014]016号)
贵州省蔬菜产业技术体系建设子项:蔬菜遗传与育种实验室建设(No.GZCYX2014-01)
关键词
白菜
游离小孢子
胚
基因型
优化
Chinese cabbage
isolated microspore
embryo
genotype
optimization
