摘要
目的:筛选与多发性骨髓瘤(multiple myeloma,MM)肾脏损害有关的血清多肽,寻找MM患者肾脏损害的早期标记物。方法:采用弱阳离子交换磁珠联合基质辅助激光解吸电离飞行时间质谱技术对临床上确诊的MM患者伴或不伴肾脏损害的血清多肽组进行对比分析。结果:在分子量为700-10 000 Da的范围内共获得18个有统计学意义的差异多肽峰(P<0.05),其中表达上调的多肽有7个,下调的有11个。利用内嵌软件将其中区分MM患者是否伴有肾脏损害的能力最强的3个质谱峰建成了1个QC(Quick Classifier)诊断模型,其敏感性为97.14%,特异性为94.12%;其中分子量为3 908.85 Da和3 216.06 Da的多肽在MM伴肾脏损害组中表达明显上调,而分子量为2 990.08 Da的多肽在MM伴肾脏损害组中明显下调。结论:利用血清多肽组学技术获得的与MM肾脏损害有关的多肽,可为临床上早期评估和诊断MM肾脏损害提供新的线索。
Objective： To screen serum peptide associated with renal impairment in patients with multiple myeloma （MM） and search early biomarker of MM renal impairment. Methods： The weak cation exchange magnetic bead combined with matrix assisted laser desorption/ionization time of flight mass spectrometry was used to compare and analyze serum peptidome of MM with or without renal impairment. Results： There were 18 peptide peaks with statistical significance in the molecular weight range from 700 to 10 000 Da（ P 〈 0. 05 ）, among them 7 peptides were upregulated and 11 were downregulated. The Quick Classifier diagnostic model composed of 3 peptides, which can strongly distinguish MM patients with or without renal impairment by means of Embedded Software. Its sensitivity and specificity were 97.14% and 94. 12%, respectively. Peptides with molecular weight of 3908. 85 Da and 3216. 06 Da were significantly upregulated in MM patients with renal impairment, while the peptide with molecular weight of 2990.08 Da was significantly downregulated in MM patients with renal impairment. Conclusion： Peptides associated with MM renal impairment obtained by serum peptidome technology can provide a new clue for early assessment and diagnosis of clinical MM renal impairment.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2017年第5期1466-1470,共5页
Journal of Experimental Hematology
基金
西安交通大学第二附属医院科研基金(科技新苗)RC(XM)201503
