摘要
土壤微生物是土壤生态功能的重要指示物,因此,研究其群落特征对指导煤矸山复垦具有重要意义。文中以山西省曹村煤矿复垦3a(R-3a)、5a(R-5a)和7a(R-7a)煤矸山为例,用磷脂脂肪酸法(PLFA)测定了3种复垦年限土壤微生物总浓度和群落浓度,分析了对土壤质量变化的敏感菌种。结果表明:1)复垦煤矸山土壤微生物总浓度和群落浓度随年限增加均有不同程度增加,表明煤矸山复垦土壤质量状况总体有改善。2)3种复垦样地微生物群落结构的差异及其总浓度和群落浓度低于普通果园(CK)的事实表明复垦地土壤质量仍难达到与CK相当的状况。3)普通细菌中i15:1 G和16:0、真菌中18:1 w9c、革兰氏阳性菌中i15:0和a15:0、阴性菌中11Me18:1 w7c和18:1 w5c可指示复垦土壤质量的改善。
Soil microbial community is an important indicator of soil ecological function. Therefore, study on soil microbial community characteristics is very significant for reclamation of coal gangue mountains. The 3a （ R - 3a）, 5a（ R- 5a） and 7a（ R- 7a） of total reclaimed soil microbial concentration and microbial community con- centration in reclamation land of coal gangue mountain in Cao village coal mine, Shanxi province, were examined by the method of phospholipid fatty acids （ PLFA）, and the indicated microbe about the change of soil quality in coal gangue mountain was analyzed. The results showed that ： （ 1 ） After the reclamation of coal gangue moun- tain, the total concentration of soil microbe and the concentration of soil microbial communities increased with the increase of reclamation years. It shows that the coal gangue mountain reclamation soil quality has improved. （2） The total soil microbe concentration and microbial community concentration were lower than ordinary orchards （CK）. It shows that reclaimed soil still lower than CK. （3） il5：lG and 16：0 IN common bacteria, 18：1 w9c in fungi, i15：0 and a15：0 in gram positive bacteria, and llMel8 ：lw7c and 18 ：lwSc in gram negative bacteria can be used as the indicators of characteristics of phospholipid fatty acid to improve soil quality conditions in coal gangue mountain reclamation.
出处
《干旱区资源与环境》
CSSCI
CSCD
北大核心
2017年第9期140-144,共5页
Journal of Arid Land Resources and Environment
基金
国家自然科学基金青年项目(41301304)资助
关键词
土壤复垦
植被恢复
微生物群落结构
指示作用
soil reclamation
vegetation rehabilitation
microbial community structure
indicative function
