摘要
目的探讨干热环境不同暴露时间大鼠肝损伤的特点及与肝组织白细胞介素-1β（IL-1β）、细胞黏附分子-1（ICAM-1）表达的相关性。方法40只雄性sD大鼠随机分为常温对照组和干热暴露组，将干热暴露组分为干热暴露50min组、100min组和150min组，每组10只。干热暴露组放置到西北特殊环境人工实验舱[环境温度（41±0．5）℃，相对湿度（10±1）％]进行热暴露，在第50、100、150分钟（时间点记为50min、100min、150min）取出相应组大鼠，测量肛温，麻醉后腔静脉取血，取肝脏后处死。常温对照组（时间点记为0min）测肛温后麻醉取血，取肝脏后处死。检测各时间点体温、肝组织病理及损伤评分、血清谷丙转氨酶（ALT）、肝组织IL-1β和ICAM-1的表达。结果随着在干热环境暴露时间延长，各指标均呈现不同程度的升高，肝病理学表现为肝细胞水肿、肝窦扩张和炎性细胞浸润、中央静脉淤血堵塞逐渐加重。各指标在50、100、150rain与前一时间点比较差异均具有统计学意义（P〈0．05）（0min与50min血清ALT水平比较，P〉0．05）。在100min大鼠体温达到41．8℃，达到中暑状态。病理评分、IL-1β、ICAM-1在100min明显升高，而ALT在150min明显升高。相关性分析发现，肝病理损伤评分与IL-1β（r=0．924，P〈0．05）、ICAM-1（r=0．975，P〈0．05）密切相关，IL-18与ICAM-1表达密切相关（r=0．943，P〈0．05）。结论干热环境的中暑早期即可出现肝病理学损伤，肝损伤程度与IL-1B、ICAM-1表达平行，内毒素引起肝窦内炎症反应，进而损伤内皮细胞引起微循环阻塞可能是导致肝损伤的原因之一；在干热环境中暑时IL-1β、ICAM-1可能是反映干热环境中暑肝损伤的早期指标，而血清ALT不能反映干热环境中暑肝脏早期损伤。
Objective To investigate the characteristics of liver injury and its correlation with interleukin - 1 beta （ IL - 1β） and intercellular adhesion molecule - 1 （ ICAM - 1 ） in rats with different exposure time in dry- heat environment. Methods Forty male SD rats were randomly divided into two groups ： normal environment control group and dry - heat environment exposure group, and the dry - heat environment exposure group was divided into three groups of 50 min, 100 rain, 150 rain according to different exposure time; each group had 10 rats. The dry - heat environment exposure groups were placed in the Simulated Climate Cabin for Special Environment of Northwest of China with the conditions of （41 ± 0.5） ℃ temperature, （ 10 ± 1 ） % relative humidity for heat exposure. In the time point of 0 min, 50 rain, 100 min, and 150 rain, the rats of corresponding groups （normal environment control group was seemed as 0 min group of heat exposure） were measured core temperature （Tc） and then anesthetized for collecting blood and liver samples for following detection including alanine aminotransferase （ALT） , IL - 1β, ICAM - 1. Histology changes of liver were observed by light microscope and pathological scores were measured. Results The indexes showed different degrees of increase with the extension of exposure time in dry - heat environment. The liver pathology showed edema of liver cells, liver sinusoidal dilatation and inflammatory cells infiltration, central venous congestion and blotting, and the extent increased gradually. Compared with the previous time point, the differences of indexes in the time point of 50 min, 100 min, 150 rain were significant （ P 〈 0.05 ） except the difference of ALT between 50 min and 0 min （P 〉 0.05 ）. In the time point of 100 min, the temperature of rats reached to 41.8 ℃ and caught heatstroke. Pathological scores, IL - 1β and ICAM - 1 increased obviously in the time point of 100 min, while ALT increased obviously in the time point of 150 min.
作者
夏亮
刘江伟
沈才福
肇寅辉
李佳佳
马娜
是文辉
Xia Liang Liu Jiang-wei Shen Cai -fu Zhao Yin-hui Li fia-jia Ma Na Shi Wen-hui.(The Key Laboratory of the Special Environment Medicine of Xinjiang, the General Hospital of Xinfiang Military Region of the PLA , Urumuqi 830000, Chin)
出处
《中国急救医学》
CAS
CSCD
北大核心
2017年第10期957-961,I0002,共6页
Chinese Journal of Critical Care Medicine
