摘要
目的通过HPLC指纹图谱结合主成分分析(PCA)对不同产地头顶一颗珠药材的质量进行评价,为其质量控制提供依据。方法采用Hibar C18色谱柱(4.6 mm×250 mm,5μm),乙腈-水为流动相进行梯度洗脱,流速为1.0 m L/min,检测波长为203 nm,柱温为30℃,利用相似度评价软件和PCA对14批头顶一颗珠药材检测结果进行分析比较。结果 HPLC指纹图谱的方法学考察符合相关要求,共标定了头顶一颗珠药材HPLC指纹图谱的15个共有峰,14批药材的相似度为0.389~0.979。PCA结果显示前3个主成分的累积方差贡献率为87.674%,S2样品综合得分最高(4.926)、质量最好。结论所建立的HPLC指纹图谱结合PCA可以客观、有效地评价不同产地头顶一颗珠药材的质量。
Objective To evaluate the quality coherence of Trillium tschonoskii Maxim. from different producing areas by HPLC fingerprint and PCA; To provide a method for quality control. Methods Samples were separated by Hibar C18（4.6 mm × 250 mm, 5 μm） with acetonitrile-water as gradient mobile phase at the flow rate of 1.0 m L/min. The wavelength was 203 nm and the temperature was 30 ℃. Chromatographic Fingerprint Similarity Evaluation System and PCA were used to analyze the data. Results The results of method validation of HPLC fingerprint met technical standards. 15 common peaks was verified and the similarities of 14 batches of Trillium tschonoskii Maxim. from different producing areas were among 0.389–0.979. 3 principal components with the characteristic root cumulative contribution rate reaching 87.674% were screened out by PCA results. The composite score of S2 was the highest（4.926）, and the quality was the best. Conclusion The application of HPLC combined with PCA can objectively and effectively evaluate the quality difference of Trillium tschonoskii Maxim. from different producing areas.
出处
《中国中医药信息杂志》
CAS
CSCD
2017年第11期58-62,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
北京市教育委员会科技发展计划面上项目(KM201510025011)
北京市属高等学校青年拔尖人才培育计划(CIT&TCD201404175)
