摘要
目的采用电感耦合等离子体质谱(ICP-MS)法测定天麻中As、Ba、Be、Cd、Co、Cr、Cu、Ga、Hg、Mg、Mn、Mo、Ni、Pb、Se、Sn、Sr、Ti、Tl、V 20种元素的量。方法采用浓硝酸与双氧水作为混合溶剂,结合微波消解处理天麻样品。经过仪器工作条件的优化,进行了方法学考察,并测定了30批不同产地天麻样品中20种元素的量。结果 20种待测元素的线性关系良好(r≥0.998 2),重复性试验RSD均≤6.34%,稳定性试验RSD均≤6.00%,精密度试验RSD均≤5.78%,加样回收率在91.62%~108.10%,RSD均≤5.21%。结论该方法具有前处理简便、多种元素同时测定、分析速度快、灵敏度高、准确度高、精密度好等优点,可为天麻中元素的快速分析以及药材质量评价提供技术支持和科学依据。
Objective To establish an ICP-MS method for determination of 20 elements in Gastrodia elata, including As, Ba, Be, Cd, Co, Cr, Cu, Ga, Hg, Mg, Mn, Mo, Ni, Pb, Se, Sn, Sr, Ti, Tl, and V. Methods Using HNO3 ＋ H2O2 as solvent, samples were digested in closed-vessel ovens by microwave. After optimization of the instrument parameters and validation of the method, contents of 20 elements in 30 batches of samples were determined. Results All the elements had good linearities（r ≥ 0.998 2） within the linear ranges, good repeatabilities（RSD ≤6.34%） and good recoveries between 91.62% and 108.10%（RSD ≤ 5.21%）. Conclusion The established method is sensitive, precious, simple and quick. It could be used for simultaneous determination of elements and as scientific basis of quality evaluation of G. elata.
出处
《中草药》
CAS
CSCD
北大核心
2017年第17期3619-3623,共5页
Chinese Traditional and Herbal Drugs
基金
江苏省中药资源产业化过程协同创新中心项目(ZDXM-1-3)
江苏高校优势学科建设工程资助项目(PAPD)
江苏高校品牌专业建设工程资助项目(PPZY2015A070)
