摘要
为研究不同工艺蒙脱石对应激性腹泻大鼠胃肠道的影响,本试验选择体重200 g左右雄性SD大鼠54只,随机分为2组,应激模型组32只,空白对照组22只。应激模型组每天灌胃番泻叶液3 m L/只·次。连续处理4 d,制作应激模型。试验第5天从应激模型组中随机取2只大鼠,对照组取2只解剖,确认应激模型。然后将应激模型组大鼠随机分为3组,分别为RT、RZ蒙脱石组和模型对照组。RT和RZ蒙脱石组每天灌胃3 m L/只·次RT型蒙脱石(钙基800目)和RZ型蒙脱石(800目钠化改性),每天2次,连续作用4 d。模型对照组灌胃相同剂量的无菌生理盐水。空白对照组1、2试验期间灌胃分别同RT和RZ型蒙脱石组。试验第5天,测定胃内pH,血清二氨氧化酶(DAO)、胃蛋白酶原Ⅰ(PGⅠ)、血清D-木糖(D-Xl)含量。结果表明:RT和RZ蒙脱石组血清D-木糖含量分别高于模型对照组13.9%和15.19%(P<0.05),分别低于空白对照组16.67%和15.35%(P<0.05);RT和RZ蒙脱石组血清DAO含量分别低于模型对照组6.28%和5.00%(P>0.05),分别高于空白对照组4.8%和4.15%(P>0.05);RT和RZ蒙脱石组小肠推进率分别低于模型对照组7.8%和8.77%(P<0.05),分别高于其空白对照组0.67%和0.73%(P>0.05)。RZ蒙脱石组血清D-木糖含量、血清DAO活性较RT蒙脱石组高1.11%和1.38%(P>0.05),RT蒙脱石组小肠推进率高于RZ蒙脱石组1.05%(P>0.05)。提示蒙脱石有修复损伤小肠黏膜细胞、调节小肠运动、提高小肠吸收的作用,而且RZ型蒙脱石效果略优于RT型蒙脱石。与模型对照组相比,RT和RZ蒙脱石组血清PG I含量分别降低9.60%和6.51%(P<0.05),胃内pH分别升高4.00%和8.00%(P<0.05)。RT和RZ蒙脱石组PG I含量分别高于各自空白对照组4.93%(P>0.05)和10.96%(P<0.05),RZ蒙脱石组PGⅠ含量高于RT蒙脱石组3.42%(P>0.05);胃内pH,RT蒙脱石组与其空白对照组无显著差异(P>0.05),RZ蒙脱石组高于其空白对照组3.85%(P<0.05),RZ蒙脱石组高于RT蒙脱石组3.85%(P<0.05)。
To observe the therapeutic effect of different processing way of montmorillonite on the model rats of stress- induced diarrhea,a total of 54 rats were divided into 2 groups including the model group with 32 rats and the control group with 22 rats.The model was structured by biding forelimbs with medical proof fabric all day after being given a gavage of 3 mL of senna infusion（1 mL was equivalent to senna 0.3 g） once everyday for 3 days.There were 4 rats being chosed to asure the success of rats modle of restraint stress.Then all the model rats were divided into 3 groups as the following：RT and RZ montmorillonite group that were respectively given a gavage of RT and RZ type montmorillonite at a dose of 0.5% of average daily feed intak twice one day.The volum of perfusion was 3 mL per rat every time.Another group was the model control group being administrated with equivalent amount of normal saline.The control group divided into 2 groups,which were RT and RZ control group.Each control group was intragastric administration just like RT or RZ montmorillonite group had being done.After 4 days,the intragaric pH,content of diamine oxidase （DAO） ,pepsinogen （PG） and D-xylose （D-XI） of serum were detected.The intestinal propulsion rate also was measured.The results showed as follows ：Compared with the model control group,the serum content of D-XI of the two montmorillonite groups increased respectively 13.9% and 15.19% （P 〈 0.05）,and the nomal control group declined respectively 16.67% and 15.35% （P 〈 0.05）.Compared with the model control group,the serum content of DAO of the two montmorillonite groups droped respectively 6.28% and 5.00% （P 〉 0.05） ,and the nomal control group rised respectively 4.8% and 4.15% （P 〉 0.05）.Compared with the model control group, intestinal propulsion rate of RT and RZ montmorillonite groups deceased respectively 7.8% and 8.77%（P 〈 0.05） ,the nomal control group increased respectively 0.67% and 0.73% （P 〉 0.05）.The serum content of D-X
出处
《中国饲料》
北大核心
2017年第20期19-22,共4页
China Feed
基金
天津农学院科学研究发展基金计划(2013N10)
关键词
应激性腹泻
蒙脱石
胃蛋白酶原
二氨氧化酶
小肠推进率
stress-induced diarrhea
montmorillonite
pepsinogen
diamine oxidase
intestinal propulsion rate
