摘要
目的探讨精神分裂症circRNA表达水平及其与患者阴性、阳性症状的关系。方法分别选取5例精神分裂症患者和正常人外周血进行基因芯片筛查,确定9种存在差异表达的circRNA,并在102例精神分裂症患者和103例正常人中进行PCR验证,进一步行精神分裂症阳性和阴性症状量表评估。结果精神分裂症患者外周血circRNA_102101、circRNA_102315、circRNA_104597、circRNA_101835、circRNA_101836的表达水平明显下调(P<0.05或P<0.01)。circRNA_103102、circRNA_103704的表达水平明显上调(P<0.01)。circRNA_102101、circRNA_103102的ΔCt值与阳性症状呈明显正相关(P<0.01或P<0.05)。circRNA_103704与阳性症状、一般精神病理呈明显正相关(P<0.01或P<0.05)。思维障碍与circRNA_102101、circRNA_103102、circRNA_102315、circRNA_103704、circRNA_102802的ΔCt值呈明显正相关(P<0.01或P<0.05)。激活性与circRNA_102101、circRNA_103102、circRNA_104597、circRNA_103704、circRNA_102802的ΔCt值呈明显正相关(P<0.01或P<0.05)。偏执与circRNA_102101、circRNA_103102、ci rcRNA_103704、ci rcRNA_102802的ΔCt值呈明显正相关(P<0.01或P<0.05)。攻击性与ci rcRNA_102101、circRNA_103102、circRNA_103704、circRNA_102802的ΔCt值呈明显正相关(P<0.01或P<0.05)。circRNA_103704进入阳性症状的回归方程(P<0.01);circRNA_103704、circRNA_102315进入一般病理表现的回归方程(P<0.01或P<0.05)。结论circRNA_103704、circRNA_103102与精神分裂症阴性、阳性症状有明显关联,可能是精神分裂症病理过程主导的调控因子。
Objective To explore the correlation of circ RNAs＇ expression level to the negative-and positive symptoms of patients with schizophrenia（SZ）. Methods Gene chip screening was performed with the peripheral blood samples from each five of SZ patients and normal controls. Nine circ RNA s show ing differentiate expression were confirmed, and further verification was done by real-time fluorescence quantitative PCR in 102 SZ patients and 103 normal controls. All the SZ patients were assessed w ith Positive and Negative Symptom Scale（PANSS）. Results It was revealed that the ex pression levels of circ RNA_102101, circ RNA_102315, circ RNA_104597, circ RNA_101835 and circ RNA_101836 were significantly down-regulated（P〈0.01 or P〈0.05）, and circ RNA_103102 and circ RNA_103704 were up-regulated in SZ group（P〈0.01）. The ΔCT value of circ RNA_102101 and circ RNA_103102 was positively correlated to the positive symptoms（P〈0.01 or P〈0.05）, and the ΔCT value of circ RNA_103704 also showed positive correlation with positive symptoms and general psychopathological symptoms（P〈0.01 or P〈0.05）. The ΔCT values of circ RNA_102101, circ RNA_103102, circ RNA_102315, circ RNA_103704 and circ RNA_102802 were correlated w ith thinking disorder（P〈0.01 or P〈0.05）, and the ΔCT values of circ RNA_102101, circ RNA_103102, circ RNA_104597, circ RNA_103704 and circ RNA_102802 were correlated with the activation（P〈0.01 or P〈0.05）. The ΔCT values of circ RNA_102101, circ RNA_103102, circ RNA_103704 and circ RNA_102802 were positively correlated with paranoid（P〈0.01 or P〈0.05）, and of circ RNA_102101, circ RNA_103102, circ RNA_103704 and circ RNA_102802 were markedly correlated with assault（P〈0.01 or P〈0.05）. Therefore, circ RNA_103704 was chosen into regressive equation of positive symptoms（P〈0.01）, and circ RNA_103704 and circ RNA_102315 were chosen into regressive equation of general pathological findings（P〈0.01 or P〈0.05）. Conclusion The expression levels of
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2017年第10期897-901,共5页
Medical Journal of Chinese People's Liberation Army
基金
南京军区医学科技创新课题(12MA002)~~
