摘要
目的 探索更好、更方便的家兔阴茎海绵体平滑肌细胞体外培养方法。方法 分别采用组织块法、酶消化法、组织块 +酶消化法对家兔阴茎海绵体平滑肌细胞进行培养 ,在倒置显微镜下对培养过程的细胞分别作了生长情况及形态学观察。用HE染色、MTT法分别描绘原代培养细胞的生长曲线、细胞分裂指数曲线 ;用倒置显微镜观察活细胞的贴壁过程 ,计数法测定细胞贴壁率。结果 组织块法及酶消化法培养的细胞其生长状况和形态学各有自己的特点。组织块法的细胞生长慢 ,培养时间相对较长 ,纯度相对较高 ,原代培养细胞生长速度快 ,但由于该法消化时间不易掌握 ,常影响其成功率 ,多数细胞呈梭形 ,细胞的密度高 ,原代培养时间为 7～ 10d。结论 每种培养方法都有各自的优缺点。
Objective To apply series of convenient and effective methods to produce large amount of pure corporal smooth muscle cells in vitro in terms of experimental requirement.Methods The explant cell culture and digestion cell culture methods were compared.Results The cell yield and purity were significantly higher in digestion cell culture compared with explant cell culture;cell growth velocity was more rapid in digestion cell culture also;but the cell culture success rates were higher in explant cell culture compared with digestion cell culture and the explant cell culture method was much easier to be mastered.Conclusions Each method had individual merit and shortcoming.The best one of the two methods could be chosen according to experimental requirement.The SMC cultured in vitro are proved to be used to evaluate and investigate the effect of some medicine on penile erection.
出处
《中国男科学杂志》
CAS
CSCD
2002年第3期197-200,共4页
Chinese Journal of Andrology
