摘要
本研究应用 PCR- SSCP银染法检测 3株人卵巢癌细胞 p5 3基因的存在状态 ,以四甲基偶唑蓝(MTT)染色法和流式细胞术 (FCM)比较不同 p5 3基因状态的肿瘤细胞在 1～ 10 Gy60 Coγ射线照射后的体外生长及凋亡率。结果显示 ,p5 3野生型的 A2 780细胞于 60 Coγ射线照射后发生明显的生长抑制和凋亡 ,而 p5 3基因突变型的 A2 780 - CP细胞和缺失型的 SKOV3细胞则呈现较强的辐射抗性。本研究结果说明 ,人卵巢癌细胞的 p5 3基因状态直接影响其对 γ射线照射的敏感性。
In the present study, the functional status of p53 in three ovarian cancer cell lines were analyzed by PCR SSCP, the differences of their proliferative capacity and apoptosis in vitro were measured respectively by MTT and cytometic analysis after 1～10 Gy 60 Co γ irradiation. The results show that A2780 cell line with wild type p53 presented a higher rate of growth inhibition and apoptosis after 60 Co γ irradiation; while A2780 with p53 mutation and SKOV3 with p53 deletion exhibited higher radioresistance in vitro. The results mentioned above indicate that the functional status of p53 gene in human ovarian cancer cell lines directly affects their sensitivities to γ irradiation. (
出处
《辐射防护》
CAS
CSCD
北大核心
2001年第6期349-353,共5页
Radiation Protection
