摘要
目的:探讨肝细胞肝癌(hepatocellular carcinoma,HCC)肿瘤微环境中CD8^+T细胞耗竭与脂代谢的关系。方法:利用流式细胞分析、实时细胞能量代谢检测仪、实时PCR等方法检测HCC和癌旁组织中CD8^+T细胞脂代谢及功能相关蛋白或基因的表达;在此基础上分析其功能和脂代谢的关系。结果:HCC浸润的CD8^+T细胞具有以下特征:功能耗竭(干扰素-γ减少、程序性死亡分子-1表达升高);多个脂肪酸合成酶表达下调,脂肪酸含量下降;线粒体结构、功能受损(P<0.05)。结论:HCC中浸润的功能耗竭CD8^+T细胞具有脂肪酸代谢异常特征。
Objective：To explore the lipid metabolic characteristics of functionally exhausted CD8^＋ T cells in tumor microenvironment of hepatocellular carcinoma（HCC）.Methods：By using flow cytometry analysis and real-time PCR to detect the functions and lipid metabolism-related proteins and genes expression of CD8^＋ T cells in HCC and peri-tumor tissues,then the relationship between function and lipid metabolism was analyzed.Results：The infiltrated CD8^＋ T cells in HCC have the following characteristics：functional exhaustion（decreased interferon-γsecretion and up regulated programmed death molecule-1 expression）;down regulated expression of multiple fatty acid synthases,and the decreased content of intracellular fatty acids;impaired mitochondrial structure and function（P0.05）.Conclusions：Fatty acid metabolism abnormalities are the characteristics of exhausted CD8^＋ T cell infiltrated in HCC.
出处
《中国临床医学》
2017年第4期514-518,共5页
Chinese Journal of Clinical Medicine
基金
上海市浦江人才计划(17PJD006)
上海市卫计委科研项目(201740101)~~
关键词
肝细胞肝癌
CD8^+T细胞
代谢
功能耗竭
微环境
hepatocellular carcinoma
CD8^＋ T cells
metabolism
functional exhaustion
microenvironment
