摘要
为了实现以水凝胶为材料的细胞支架快速加工,将计算全息法引入传统的飞秒激光双光子加工中,并对全息图的生成方法和全息图对加工结构的影响进行了研究。首先,根据贝塞尔光波动方程和其透射函数生成贝塞尔光束全息图,分析两种参数对环形结构大小和质量的影响。然后利用生成的全息图加工得到壁厚800nm、直径为8~15μm不等的水凝胶(PEGDA)圆管结构。最后,实现了基于圆管道的水凝胶细胞支架高效快速加工,支架中圆管道壁厚800nm、直径为8μm。本文首次将飞秒激光全息加工技术应用于水凝胶三维支架加工,解决了飞秒激光单点加工效率的问题。该技术在生物医学中具有广阔的应用前景。
In order to achieve rapid fabrication of hydrogel cell scaffolds,the computer-aided holographic method was employed into the traditional femtosecond laser two-photon processing.The generation of hologram as well as the influence of its parameters on the processed cylinder microstructure was studied.Firstly,Ring-shaped Bessel-beam holograms were generated according to the Bessel light wave equation and its transmission function,of which the parameterseffect on the size and quality of ring structure was analyzed.Then the generated holograms were used to process tubes with a thickness of 0.8 μm and diameters from 8 μm to 15 μm in the polyethylene glycol double acrylate（PEGDA）hydrogel.Finally,an efficient and rapid processing of hydrogel cell scaffolds was realized experimentally and the diameter of hydrogel tubes was 8 μm.This technique can remarkably improve the efficiency of femtosecond laser processing,thus has great potential in many applications such as biological culture and chemical analyses.
出处
《光学精密工程》
EI
CAS
CSCD
北大核心
2017年第9期2277-2282,共6页
Optics and Precision Engineering
基金
国家自然科学基金资助项目(No.51675503
No.61475149
No.51405464
No.61675190
No.51605463)
中央高校基本研究经费资助项目(No.WK2480000002)
中国博士后科学基金资助项目(No.2016M590578
No.2016M602027)
中科院仪器研制项目(No.YZ201566)
