摘要
目的了解新疆柯尔克孜族人各种(类)红细胞血型系统抗原基因的多态性分布情况。方法采用PCRSSP方法对113名新疆柯尔克孜族无血缘关系的健康献血者Kell、Duffy、Diego、Kidd、Dombrock、Yt和Colton 7个血型系统做基因分型,并做基因多态性分析。结果本组新疆柯尔克孜族无偿献血者人群的Kell、Duffy、Diego、Kidd、Dombrock、Yt和Colton7个血型系统抗原基因频率分别为K=0.008 8(2/226)、k=0.991 2(224/226),Jsa=1.000 0(226/226)、Jsb=0.000 0(0/226),Fya=0.725 7(164/226)、Fyb=0.274 3(62/226),Dia=0.057 5(13/226)、Dib=0.942 5(213/226),Jka=0.615 0(139/226)、Jkb=0.385 0(87/226),Doa=0.238 9(54/226)、Dob=0.761 1(172/226),Yta=0.946 9(214/226)、Ytb=0.053 1(12/226),Coa=1.000 0(226/226)、Cob=0.000 0(0/226),均符合Hardy-Weinberg遗传定律(P>0.05),Kell和Colton基因分布与汉族、维吾尔族、回族、藏族、满族人群相似。结论新疆柯尔克孜族人Duffy、Diego、Kidd、Dombrock和Yt血型系统基因频率具有独特的分布特点,Kell和Colton血型系统与国内许多民族相似;Colton血型系统基因呈单态性分布。
Objective To investigate gene polymorphism of Kell, Duffy, Diego, Kidd, Dombrock, Yt, and Colton blood group systems in Xinjiang Khalkhas. Methods The genotypes of seven blood groups of 113 samples from unrelated healthy Xinjiang Khalkhas blood donors were analyzed by polymerase chain reaction-sequence specific primer（PCR-SSP） .Results The gene frequencies of seven blood group systems were as follows ：K = 0. 008 8 （2/226）, k = 0. 991 2 （224/226）, Js^a= 1. 000 0 （ 226/226）,Js^b = 0. 000 0 （0/226）, Fy^a = 0. 725 7 （ 164/226）, Fy^b = 0. 274 3 （62/226）, Di^a = 0. 057 5 （13/226）, Di^b = 0. 942 5（213/226） ,Jk^a = 0. 615 0（139/226） ,Jk^b = 0. 385 0（87/226） ,Do^a = 0. 238 9（54/226） ,Do^b = 0. 761 1 （172/226）, Yt^a = 0. 946 9（ 214/226）, Yt^b= 0. 053 1 （ 12/226）, Co^a = 1. 000 0（ 226/226）, Co^b = 0. 000 0（172/226） .There was a good fit to Hardy-Weinberg equilibrium within each group. Conclusion The frequency distribution of Duffy, Diego, Kidd, Dombrock and Yt blood groups in Xinjiang Khalkhas has its unique characteristics. Kell and Cohon have similar distribution with Han, Xinjiang Uygur, Hui, Tibet, Manchu people in China.The gene distribution of Colton is monomorphic.
出处
《中国输血杂志》
北大核心
2017年第8期931-934,共4页
Chinese Journal of Blood Transfusion
基金
新疆维吾尔自治区自然科学基金(2015211A009)
