摘要
目的制备粗毛豚草素脂质体,优化其处方并评价其体外抗肿瘤作用。方法采用薄膜分散法制备粗毛豚草素脂质体,对脂质体的粒径、Zeta电位进行表征;并采用磺酰罗丹明B蛋白(SRB)法考察脂质体对A549细胞的抗增殖作用。结果采用最优处方,制备的目标脂质体中粗毛豚草素的包封率为(87.06±0.6)%;平均粒径为(104.83±1.40)nm;Zeta电位为(-4.61±0.32)mV。制备得到的粗毛豚草素脂质体对A549细胞具有较强的抑制作用。结论本实验组制备脂质体的方法简单易行,脂质体粒径均一,包封率较高且体外抗肿瘤作用较强。
OBJECTIVE To study the preparing methods of hispidulin liposomes and evaluate their antitumor effects in vitro. METHODS Hispidulin liposomes were prepared by thin film dispersion method, and the formulation was further optimized. At the same time, the liposomes particle size and Zeta potential were characterized separately. The antiproliferative effect of the liposomes on A549 cells was investigated by sulforhodamine B （SRB） method. RESULTS According to the optimal prescription, the encapsulation efficiency of hispidulin liposomes was （87.06±0. 67） %. Additionally, the average particle size was （ 104. 83±1.40） nm and the Zeta potential was （ -4. 61±0. 32） mV. Furthermore, the hispidulin liposomes exhibited the strongest inhibiting effect on A549 cells in vitro with comparison with control groups. CONCLUSION The preparation method buihin our research is easy. And the liposomes are well distributed with high encapsulation efficiency. Meanwhile, the hispidulin liposomes exhibit the most significant antitumor effects in vitro.
作者
李霞
应雪
闫荷露
王亚华
徐浩伦
李兆萍
LI Xia YING Xue YAN He-lu WANG Ya-hua XU Hao-lun LI Zhao-ping(Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, Chin)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2017年第18期1624-1628,共5页
Chinese Pharmaceutical Journal
基金
国家自然科学基金资助项目(81760421
81460540)
新疆生产建设兵团社会发展科技攻关与成果转化计划资助项目(2015AD007)
