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4种中草药提取液的工艺优化及其抗菌效果对比 被引量:11

Optimization of 4 kinds of Chinese herbal extracts and comparative study on their antibacterial activity
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摘要 目的研究金银花、甘草、胖大海、薄荷4种中草药提取的最佳工艺,并为制备清咽润喉凉果做基础。方法以单因素实验考察提取温度、料液比、提取时间对4种中草药提取液的影响,以抑菌圈直径来评价抗菌能力。在单因素基础上,设计正交优化试验,得到4种中草药的最佳提取工艺。结果金银花的最佳提取条件为温度80℃、料液比1:30(g:mL)、时间3h;甘草的最佳提取条件为温度70℃,料液比为1:30(g:mL),时间为5h;胖大海的最佳提取条件为:温度60℃,料液比1:20(g:mL),时间3h;薄荷的最佳提取条件为:温度70℃,料液比1:30(g:mL),时间3h。结论本研究得出4种中草药的最佳提取工艺,而且得出的中草药提取液具有一定抑菌性可用于制作清咽润喉凉果。 Objective To develop the optimum extract techniques of 4 kinds of Chinese herbs, including Lonicerae japonicae, Glycyrrhizae radix, Sterculia lychnophora Hance and Herba Menthae, for the preparation of clearing and moistening laryngopharynx preserved fruit. Methods The effects of extraction temperature, ratio of material to liquid and extraction time on 4 kinds of Chinese herb extracts were investigated using the diameter of inhibition zone as index. On the basis of single factor experiment, orthogonal experiment was designed to obtain the optimum extraction techniques of the 4 kinds of Chinese herbs. Results The optimum extraction conditions of Lonicerae japonicae were as follows: temperature was 80 , ratio of solid to liquid℃ was 1:30 (g:mL), and extraction time was 3 h. The optimum extraction conditions of Glycyrrhizae radix were that the temperature was 70 , ratio of℃solid to liquid was 1:30 (g:mL), and extraction time was 5 h. The optimum extraction conditions of Sterculia lychnophora Hance were as follows: the temperature was 60 ℃ and extraction time was 5 h with the ratio of solid to liquid of 1:20 (g:mL). The optimal conditions for the extraction of Herba Menthae were as follows: the temperature was 70 , ratio of material to liquid℃ was 1:30 (g:mL), and extraction time was 3 h. Conclusion The results obtain the optimum extract techniques of 4 kinds of Chinese herbs, and the extracts can be used for preparing for clearing and moistening laryngopharynx preserved fruit.
作者 胡燕敏 周爱梅 宋倩 方琼谜 李雪娜 林春秀 HU Yan-Min ZHOU Ai-Mei SONG Qian FANG Qiong-Mi LI Xue-Na LIN Chun-Xiu(College of Food, South China Agricultural University, Guangzhou 510640, China)
出处 《食品安全质量检测学报》 CAS 2017年第8期3169-3176,共8页 Journal of Food Safety and Quality
基金 华南农业大学大学生创新创业训练计划项目 广东省省部产学研结合项目(2013B090600003)~~
关键词 中草药 提取 金银花 甘草 胖大海 薄荷 抑菌 Chinese herbal extraction Lonicerae japonicae Glycyrrhizae radix Sterculia lychnophora Hance Herba Menthae antibacteria
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