摘要
为了获得大果、质优的多倍体品种,以猕猴桃‘琼露’无菌苗叶柄为材料,分别利用抽滤灭菌的0.05%、0.10%的秋水仙素溶液浸泡3、4、5 h,培养再生植株,利用流式细胞仪进行倍性鉴定。结果表明:0.05%的秋水仙素处理3 h,存活率和再生频率分别达到85.19%和82.61%,低于对照;当0.10%的秋水仙素处理5 h时,其存活率和再生频率分别降低至36.00%和44.44%。其中,以0.05%的秋水仙素处理4 h诱导效果最佳,变异率为28%。倍性鉴定结果表明,共获得四倍体植株7株,嵌合体5株。多倍体与二倍体相比,叶片变大,叶面变厚,节间变短,与二倍体差异显著。
In order to obtain some polyploidy varieties with big fruit and good quality, using the petioles of ＇Qionglu＇ kiwifruit as materials, the materials were respectively treated by 0.05% and 0.10% eolehicine sterilized by filtration for 3, 4, 5 h, regeneration plants were cultivated, and ploidy was identified by using flow cytometry. The results showed that： the survival rate and regeneration rate respectively reached 85.19% and 82.61% in the treatment of 0.05% colehicine for 3 h, lower than that of the control; in the treatment of 0.1% colchicine for 5 h, the survival rate and regeneration rate were reduced to 36% and 44.44%, respectively. Among them, the treatment of 0.05% colchicine for 4 h had the best effect of induction, and the variation rate was 28%. The results of ploidy identification showed that total 7 tetraploid plants were obtained, and total 5 mixoploid plants were obtained. Compared with the diploid plants, the leaves in the polyploidy plants became larger and thicker, distances of internodes became shorter, and the differences were significant.
出处
《经济林研究》
北大核心
2017年第3期147-151,共5页
Non-wood Forest Research
基金
中国农业科学院科技创新工程专项(CASS-ASTIP-2017-ZFR1)
河南省现代农业产业技术体系项目(S2014-11)
中央级科研院所基本科研业务经费专项(1610192016303
1610192016616)
