摘要
目的探讨早期糖尿病肾病(diabetic nephoropathy,DN)患者血清中性粒细胞明胶酶相关脂质运载蛋白(neu-trophil gelatinase-associated lipocalin,NGAL)与超敏C反应蛋白(high-sensitivity C-reaction protein,hs-CRP)、血清胱抑素C(cystatian C,Cys C)、尿白蛋白(urine-microalbumin,U-m ALB)的改变及其对DN早期诊断的临床意义。方法将2型糖尿病(type 2 diabetes mellitus,T2DM)患者156例按尿液清蛋白排泄率(urinary albumin excretion rate,UAER)分为三组:单纯糖尿病组(NA组)、早期肾病组(MA组)、临床肾病组(CN组);同时采用50例健康体检者作为正常对照组(NC组)。通过采用酶联免疫吸附法(ELISA)检测各组血中的NGAL浓度,乳胶增强免疫比浊法检测hs-CRP,免疫速率散射比浊法检测Cys C,化学发光法检测U-m ALB。对检测数据采用SPSS统计软件进行统计学分析。结果血清NGAL NA组、MA组、CN组显著高于NC组,差异有统计学意义(P<0.05);但MA组与CN组间比较,差异无统计学意义(P>0.05)。与NC组比较,NA组、MA组、CN组hs-CRP、Cys C、U-m ALB水平显著升高,差异有统计学意义(P<0.01)。血清NGAL与hsCRP、Cys C、U-m ALB均呈正相关(R2=0.281 1、0.180 9、0.858 0,P<0.001)。结论 T2DM早期肾损伤血清NGAL水平升高迅速,是临床诊断早期DN敏感而准确的指标,血清NGAL联合hs-CRP、Cys C、U-m ALB检测具有较高的临床诊断价值。
Objective To investigate the changes of serum neutrophil gelatinase-associated lipocalin （ NGAL ） , high-sensitivity C-reaction protein （ hs-CRP ） , cystatian C （ CysC ） and urine-microalbumin （ U-mALB ） expression in patients with early diabetic nephropathy （DN） and their clinical significance. Methods One hundred and fifty-six cases of type 2 diabetes mellitus （ T2DM ） were divided into three groups according to the urine albumin excretion rate （ UAER） , simple diabetic group （ NA ） , early diabetic nephropathy group （ MA ） and clinical nephropathy group （ CN ）. Fifty healthy physical examinees were recruited as control group （ NC）. Serum NGAL was measured by ELISA. Serum hs-CRP were determined by latex-enhanced immunot-urbidimetry. Serum CysC was detected by rate immune scatter turbidimetry. Serum U-mALB was detected by chemiluminescent. The data were statistically analyzed by SPSS software. Results Serum NGAL was higher in NA, MA and CN group than in NC group （ P 〈0.05） , but was not significantly different between MA group and CN group （ P〉0.05）. Moreover, the serum levels of hs-CRP, CysC and U-mALB were higher in NA, MA and CN groups than in NC group（ P〈0.05 ）. Serum NGAL were positively correlated with hs-CRP, CysC and U-mALB （R^2=0.2811, R^2 =0.1809 and R^2= 0.8580 respectively, all P〈0.001）. Conclusions Serum NGAL is rapidly increased in early diabetic nephropathy. It is a sensitive and accurate index. Combination of NGAL with hs- CRP, CysC and U-mALB has higher clinical significance in diagnosis of early diabetic nephropathy.
作者
黄炎
黄伟
章爽
姜锐
朱建华
HUANG Yah HUANG Wei ZHANG Shuang JIANG Rui ZHU Jian-hua(Department of Clinical Laboratory, Wuhan NO. 1 Hospital, Wuhan, Hubei 430022, China)
出处
《实用预防医学》
CAS
2017年第10期1168-1171,共4页
Practical Preventive Medicine
基金
2014国家高技术研究发展计划(863计划)(编号:AA022304)
