摘要
研究证明,转录因子广泛参与植物的生长发育过程并响应多种生物/非生物胁迫信号途径。低温胁迫可导致紫花苜蓿(Medicao sativa)减产、越冬率降低以及生产年限缩短。利用新一代高通量转录组测序技术,本研究对4℃低温胁迫下紫花苜蓿中响应低温胁迫的转录因子基因进行了鉴定,并对其表达情况进行分析,结果表明,转录组测序共获得78 925条Unigene序列和3 448个差异表达基因。其中,从3 448个差异表达基因中共鉴定出43个转录因子家族,共251个基因被显著地诱导表达。不同转录因子家族基因受低温胁迫诱导表达不同。本研究有助于在整体水平加深了解紫花苜蓿转录因子表达特性,为进一步研究这些响应低温胁迫的转录因子的功能提供参考。
Numerous studies have shown that transcription factors are important in regulating plant develop- ment and responses to various biotic/abiotic stressors. Low temperature stress could result in lowered produc- tion, reduced overwintering rate and a decrease in sustainability. In the present study, the identification and gene expression model of the transcription factors in alfalfa under low temperature stress （4℃） were analysed by the high-through put RNA-Seq technology. The results showed that a total of 78 925 Unigene sequences and 3 448 differently expressed genes were produced in our study. There were 43 transcription factor families, and a total of 251 genes were identified from all differently expressed genes. The expression model of these differ- ently expressed transcription factor genes was different. This study facilitates the understanding of the expres- sion model and verifies the related function of these low temperature response transcription factor genes in alfalfa.
出处
《草业科学》
CAS
CSCD
北大核心
2017年第9期1824-1829,共6页
Pratacultural Science
基金
现代农业产业技术体系建设专项资金国家牧草产业技术体系项目(CARS-35)
中国农业科学院科技创新工程牧草栽培与加工利用团队项目(CAAS-ASTIP-IGR 2015-02)
国家自然科学基金青年科学基金(31502000)
兰州大学中央高校基本科研业务费专项资金(lzujbky-2016-8)
国家草品种区域试验项目(21301060001)
巴彦淖尔肉羊优质饲草高效生产关键技术集成与研究应用
关键词
紫花苜蓿
转录因子
低温胁迫
UNIGENE
转录组
Medicago sativa
transcription factors
low temperature stress
Unigene
transcriptome
