摘要
目的观察银杏叶提取物(EGb)预处理对2型糖尿病大鼠心肌梗死后心血管保护的效应与机制。方法采用链脲佐菌素腹腔注射(35 mg/kg)加高脂饲料喂养建立2型糖尿病大鼠模型,将模型大鼠随机分为模型组(蒸馏水)、假手术组(蒸馏水)、二甲双胍组[100 mg/(kg·d)]、EGb低剂量组[200 mg/(kg·d)]及EGb高剂量组[400 mg/(kg·d)],每组12只,连续灌胃4周后前降支结扎模拟大鼠心肌梗死状态,另选取10只作为正常组。观察各组大鼠空腹血糖(FBG)、血清胰岛素(FINS)、胰岛素抵抗指数(IRI)及胰岛素敏感指数(ISI)、TG、TC、HDL-C、LDL-C,血浆P-选择素(P-selectin)、CK-MB、c Tn I、TNF-α、IL-1、IL-6、凝溶胶蛋白(gelsolin)、肌动蛋白微丝(F-actin)及维生素D结合蛋白(DBP)水平。结果与正常组比较,模型组及假手术组大鼠FBG、FINS、IRI、TG、TC、LDL-C、CK-MB、CTn I、IL-6、TNF-α、IL-1、P-选择素、gelsolin(PRP)、F-actin升高(P<0.01,P<0.05),ISI、HDL-C、gelsolin(PPP)、DBP降低(P<0.01,P<0.05)。与模型组比较,各给药组FBG、FINS、IRI、TG、TC、LDL-C、CK-MB、CTn I、IL-6、TNF-α、IL-1及P-选择素降低(P<0.01,P<0.05),ISI、HDL-C升高(P<0.01,P<0.05);EGb两个剂量组gelsolin(PRP)、F-actin降低(P<0.05),gelsolin(PPP)、DBP升高(P<0.05)。结论EGb具有降糖、调脂、抗炎及改善胰岛素抵抗的作用,预处理能够减轻因急性心肌缺血导致的心肌损伤,其心血管保护效应可能与激活肌动蛋白清除系统密切相关。
Objective To observe the effect and mechanism of extract of Ginkgo biloba（ EGb） on cardiovascular protection on the rat model of type 2 diabetes（ T2DM） after myocardial infarction. Methods T2 DM rat model was established by intraperitoneal injection of streptozotocin（ 35 mg/kg） combined with high fat diet,and the model rats were randomly divided into the model group（ equivalent distilled water）,the sham group（ equivalent distilled water）,the metformin group（ 100 mg·kg^（-1）·d^（-1））,the low dose of EGb group（ 200 mg·kg^（-1）·d^（-1）） and the high dose of EGb group（ 400 mg·kg^（-1）·d^（-1））,12 in each group. The myocardial infarction rat model was established by ligating the anterior descending branch of coronary artery after T2 DM rats were administered with EGb for 4 weeks continuously. Besides,another 10 rats were recruited as a normal group. The levels of fasting blood glucose（ FBG）,fasting serum insulin（ FINS）,insulin resistance index（ IRI） and insulin sensitive index（ ISI）,TG,TC,HDL-C andLDL-C,plasma P-selectin,CK-MB and c Tn I,TNF-α,IL-1 and IL-6,gelsolin,F-actin and vitamin D binding protein（ DBP） were observed. Results Compared with the normal group,FBG,FINS,IRI,TG,TC,LDL-C,CK-MB,CTn I,IL-6,TNF-α,IL-1,P-selectin,gelsolin（ PRP）,F-actin increased（P 0. 01,P 0. 05）,ISI,HDL-C,gelsolin（ PPP）,DBP decreased in each medicated group（P 0. 01,P 0. 05）. Compared with the model group,FBG,FINS,IRI,TG,TC,LDL-C,CK-MB,CTn I,IL-6,TNF-α,IL-1,P-selectin decreased（P 0. 01,P 0. 05）,ISI,HDL-C increased in the EGb group and metformin group（P 0. 01,P 0. 05）; gelsolin（ PRP）,F-actin decresed（P 0. 05）,gelsolin（ PPP）,DBP increased in the two EGb treated group（P 0. 05）. Conclusions EGb could reduce blood glucose,regulate lipid metabolism,anti-inflammatory and improve insulin resistance. EGb pretreatment could relieve myocardial injury caused by acute myocardial ischemia,and its cardiovascular protective effect may be r
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2017年第9期1100-1104,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
北京市科技新星计划资助项目(No.Z171100001117027)
北京市优秀博士学位论文指导教师科技项目(No.20138450201)
关键词
银杏叶提取物
2型糖尿病
心肌梗死
肌动蛋白清除系统
extract of Ginkgo biloba
type 2 diabetes
myocardial infarction
actinscavenger system
