摘要
目的:建立同时测定三棱-莪术药对中P-香豆酸、阿魏酸含量的方法,探讨不同比例药对中二者含量的变化。方法:采用高效液相色谱法。色谱柱为Agilent 20RBAX XDB-C_(18),流动相为乙腈-0.1%冰醋酸溶液(梯度洗脱),流速为1.0 m L/min,检测波长为266 nm,柱温为30℃,进样量为10μL。结果:P-香豆酸、阿魏酸检测质量浓度线性范围分别为4.218 6~21.093μg/m L(r=0.999 8)、1.836 0~9.180μg/m L(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为98.72%~100.30%(RSD=0.18%,n=9)、99.11%~100.45%(RSD=0.46%,n=9)。三棱-莪术药对4个配伍比例(1∶1、2∶1、1∶2、1∶0,m/m)中,三棱-莪术比例(m/m)为2∶1时P-香豆酸、阿魏酸含量最高。结论:该方法操作简便,精密度、稳定性、重复性好,可用于三棱-莪术药对中P-香豆酸、阿魏酸含量的同时测定;该药对中三棱-莪术比例(m/m)为2∶1时主要有效成分含量最高。
OBJECTIVE： To establish a method for simultaneous determination of P-coumaric acid and ferulic acid in the cou- ple of Sparganium stoloni and Curcuma aeruginosa, and to investigate rational proportion of the couple. METHODS： HPLC meth- od was adopted. The determination was performed on Agilent 20RBAX XDB-C18 column with mobile phase consisted of acetoni- trile-0.1% phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 266 nm, and the column temperature was 30℃. The sample size was 10 μL. RESULTS： The linear ranges of P-coumaric acid and ferulic acid were 4.218 6-21.093 μg/mL（r=0.999 8）, 1.836 0-9.180 μg/mL（r=0.999 9）, respectively. RSDs of precision, stability and reproduc- ibility tests were all lower than 2.0%. The recoveries were 98.72%-100.30% （RSD=0.18%, n=9）, 99.11%-100.45% （RSD= 0.46 %, n = 9）. Among 4 compatibility proportions of the couple of S. stoloni and C. aeruginosa （ 1 ： 1,2 ： 1,1 ： 2,1 ： O, m/m）, the dis- solution rate of P-coumaric acid and ferulic acid were the highest when the ratio of S. stoloni-C, aeruginosa （m/m）was 2： 1. CON- CLUSIONS： The method is simple, accurate, stable and reproducible, and can be used for content determination of P-coumaric ac- id and ferulic acid in the couple of S. stoloni and C. aeruginosa, when the proportion of the couple of S. stoloni and C. aeruginosa is 2：1 and the contents of P-coumaric and ferulic acid are the highest,the contents of main effective components are the highest.
出处
《中国药房》
CAS
北大核心
2017年第27期3859-3861,共3页
China Pharmacy
基金
佳木斯大学基础研究类项目(No.JMSUJCMS2016-035)
关键词
高效液相色谱法
三棱-莪术
药对
P-香豆酸
阿魏酸
含量测定
HPLC
Sparganium stoloni-Curcuma aeruginosa
Couple
P-coumaric acid
Ferulic acid
Content determination
