摘要
建立高效液相色谱法测定白花蛇舌草中对香豆酸和芦丁含量的方法。色谱柱:Kromasil C18(250 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸溶液(30∶70);流量:1.0 mL/min;对香豆酸和芦丁的检测波长分别为308 nm和353 nm;柱温:30℃。对香豆酸的质量浓度在2.11~12.66μg/mL范围内与其色谱峰面积具有良好的线性关系(r=1.0000);芦丁的质量浓度在8.34~50.02μg/mL范围内与其色谱峰面积具有良好的线性关系(r=1.0000)。对香豆酸、芦丁的平均回收率分别为94.01%、91.05%。对香豆酸、芦丁6次测定结果的相对标准偏差分别为1.60%、1.16%。该方法可用于同时测定白花蛇舌草中对香豆酸和芦丁含量。
The method for determination of p-Coumaric acid and Rutin in Oldenlandia Diffusa(Willd.)Roxb.by HPLC was established.The chromatographic column was Kromasil C18(250 mm×4.6 mm,5μm)and the column temperature was 30℃.The mobile phase was methanol-0.1%H3PO4 water solution(volume ratio was 30∶70)with the flow rate of 1.0 mL/min.The detection wavelengths of p-Coumaric acid and Rutin were 308 nm and 353 nm,respectively.The mas concentration of p-Coumaric acid had good linear relationship with the chromatographic peak area in the range of 2.11-12.66μg/mL with r=1.0000.The mas concentration of Rutin had good linear relationship with the chromatographic peak area in the range of 8.34-50.02μg/mL with r=1.0000.The average recovery of p-Coumaric acid and Rutin were 94.01%and 91.05%,respectively.The relative standard deviations of determination results for p-Coumaric acid and Rutin were 1.60%,1.16%(n=6),respectively.The method is suiatble for the simultaneous determination of p-Coumaric acid and Rutin in Oldenlandia diffusa(Willd.)Roxb.
作者
齐红
于姗姗
李敏
张秋红
Qi Hong;Yu Shanshan;Li Min;Zhang Qiuhong(Jinan Institute for Food and Drug Control,Jinan 250102,China)
出处
《化学分析计量》
CAS
2021年第6期57-61,共5页
Chemical Analysis And Meterage
基金
山东省中医药管理局2017-2018中医药科技发展项目(2017-313)
山东省药品监督管理局山东省中药材及饮片标准研究(2020-021)。
关键词
白花蛇舌草
对香豆酸
芦丁
高效液相色谱法
Oldenlandia Diffusa(Willd.)Roxb.
p-Coumaric acid
Rutin
high performance liquid chromatography
