摘要
目的:探讨转化生长因子β1(TGF-β1)对人牙周膜干细胞(h PDLSCs)增殖和骨向分化的影响。方法:将体外培养的h PDLSCs随机分为对照组(不含TGF-β1)和2个实验组(1、10 ng/mL TGF-β1),用CCK8检测细胞的增殖情况;碱性磷酸酶(ALP)活性、茜素红染色及实时定量PCR检测各组细胞的成骨分化情况。结果:TGF-β1可促进细胞增殖,且10 ng/mL组细胞的增殖能力较强(P<0.05);与对照组相比,实验组的ALP活性明显升高,且10 ng/mL组较1 ng/mL组升高更明显(P<0.05);茜素红染色结果显示,实验组h PDLSCs染色显著,矿化结节形成范围大且呈深橘色,10 ng/mL组较1 ng/mL组染色更显著;RT-PCR结果显示,与对照组相比,10 ng/mL组成骨相关基因牙骨质附着蛋白(CAP)、骨桥蛋白(OPN)、骨钙蛋白(OCN)、Runt相关转录因子2(Runx2)表达量明显升高(P<0.05)。结论:10 ng/mL的TGF-β1可明显促进h PDLSCs的骨向分化。
AIM To evaluate the effects of TGF-β1 on the osteogenesis of human periodontal ligament stem cells （hPDLSCs）. METHODS： hPDLSCs were in vitro cultured with TGF-β1 at 0 ng/mL （control group） , 1 ng/mL and 10 ng/mL respectively. CCK8 assay, alkaline phosphatase（ ALP） activity assay, alizarin red staining and real time quantitative PCR were conducted for the evaluation of the osteogenesis of hPDLSCs. RESULTS： TGF-β1 promoted cell proliferation, and 10 ng/mL TGF-β1 was the most effective. ALP in the experimental groups was higher than in the con-trol group, in 10 ng/mL group was higher than in 1 ng/mL group （P 〈 0.05 ） . Mineralized nodule formation in the exper-imental groups was significantly more than in the control group, and in 10 ng/mL group was more than in 1 ng/mL group. The mRNA expression of osteopontin（ OPN） , osteocalcin （ OCN ） , Runt - related transcription factor 2（ RUNX-2） and Cementum Attachment Protein（ CAP） were significantly increased in 10 ng/mL TGF-β1 group（ P 〈 0. 05 ） . CONCLU-SION： TGF-β1 is a potent regulator of osteogenesis differentiation of human periodonal ligament cells.
出处
《牙体牙髓牙周病学杂志》
CAS
2017年第8期437-441,共5页
Chinese Journal of Conservative Dentistry
基金
新疆维吾尔自治区高校科研计划青年教师科研启动基金(XJEDC2016S055)
