摘要
【目的】通过观测转复合多基因欧美杨107杨(转基因107杨)幼苗在不同浓度盐胁迫下生长性状,质膜透性、光合作用等各项指标,以及外源基因表达产物积累量的变化,综合评价转复合多基因杨树在盐胁迫下的适应能力。【方法】以转化成功且已经过验证的转基因107杨为试验材料,未转基因107杨为对照(CK),进行组培扩繁,在幼苗株高10 cm时定植在花盆中,置于人工气候室中培养。分别用0、3、6 g·L^(-1)的Na Cl溶液进行盐胁迫处理,胁迫25天后观测2个株系幼苗的生长指标、生理参数变化,以及甜菜碱与Bt毒蛋白的积累量。【结果】2个株系的生长指标观测结果表明:在低盐浓度处理下,转基因107杨幼苗株高增长量显著高于非转基因对照,转基因株系幼苗地径增长量与对照差异不显著;高盐浓度处理下,2个株系幼苗株高与地径均受到盐胁迫的显著影响。2个株系的生理参数观测结果表明:在低盐浓度处理下,转基因107杨幼苗质膜透性显著低于对照107杨,叶绿素含量有一定程度的增加,净光合速率与蒸腾速率显著高于对照,光系统Ⅱ实际光合效率Y(Ⅱ)小幅度降低但显著高于对照,光系统Ⅱ能够保持较高的电子传递速率;F_v/F_m增幅较高,具有较大光合作用潜力。在高盐浓度下,转基因株系幼苗叶绿素含量降幅较小,而质膜透性、净光合速率均显著降低,F_v/F_m、Y(Ⅱ)均呈现较大幅度下降,光系统Ⅱ电子传递速率受到较大影响,光合能力下降,变化趋势与对照相同,2个株系幼苗均受到较大程度的盐害。转基因株系中外源基因表达产物积累量的ELISA研究结果表明,随盐浓度的增加,Cry1Ac毒蛋白、Cry3A毒蛋白及甜菜碱含量都呈现增加趋势;在高盐浓度胁迫下外源基因产物积累量均显著增加。【结论】转基因107杨在低浓度盐胁迫下表现出良好的适应性,耐盐性优于对照107杨。在高浓度盐胁迫下转基因
【Objective 】 This paper aimed to comprehensively evaluate the adaptability of transgenic Populus ×euramericana cv. ‘74/76 ＇ with multiple genes（ Abbreviation： Transgenic poplar 107） under salt stress through measurements of growth traits,membrane permeability and photosynthesis and other physiological parameters of the transgenic polar 107 under different salt stresses,as well as of the change in the accumulation amount of exogenous gene expression products. 【Method】The transgenic polar 107 was used as test material,which had been verified successful transformation. The transgenic materials were propagated by tissue culture, and then the obtained seedlings were transplanted in pots when their heights were up to 10 cm. The seedlings were cultured in growth chamber and treated with Na Cl solutions which concentrations were 0,3,and 6 g· L^（-1）,with the nontransgenic poplar 107 served as the control（ CK）. The growth indexes and physiological parameters,as well as accumulation of betaine and Bt toxin proteins of two lines were measured in 25 days after treatment. 【Result】The results showed that under low salt concentration treatment,the height of transgenic poplar 107 was significantly higher than that of poplar 107,and the diameter of the transgenic lines was greater than that of poplar 107. Under high salt concentration,the height and the ground diameter of the seedlings of the two lines all were significantly affected by salt stress. Under low salt concentration,membrane permeability of transgenic poplar 107 was significantly lower than that of polar 107,chlorophyll content increased more greatly,and both net photosynthetic rate and transpiration rate increased and were significantly higher than that of polar 107. The actual photosynthetic efficiency Y（ Ⅱ） of photosystem Ⅱ decreased slightly,but significantly higher than that of the control,indicating that photosystem Ⅱ was able to maintain higher electron transfer rate. F_v/F_mhad a relatively greater increase,suggesting i
出处
《林业科学》
EI
CAS
CSCD
北大核心
2017年第7期45-53,共9页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(31370663)
国家高技术研究发展计划("863"计划)项目(2013AA102703)
关键词
转复合多基因
欧美杨107杨
耐盐性
BADH基因
BT基因
transgenic with multiple genes
Populus × euramericana cv. ‘74/76 ＇
salt tolerance
BADH gene
Bt gene
