摘要
目的:采用不同方式对土鳖虫酶解液进行脱盐操作,优选最佳脱盐方式和工艺。方法:以脱盐率、肽损失量、固体物质保留量为指标,分别采用大孔树脂方法、阴阳离子混合树脂方法、纳滤方法、电渗析方法对土鳖虫酶解液进行脱盐实验,并对最佳方式进行工艺优化,得到最优脱盐工艺。结果:采用大孔树脂、阴阳离子混合树脂、纳滤、电渗析4种方法对土鳖虫酶解液进行脱盐处理,得出4种方法的脱盐率分别为95.19%、60.97%、90.01%、94.43%。其中4种方法造成的肽损失率和固体物质保留率分别为32.12%、20.91%、40.55%、10.12%;50.43%、80.45%、52.76%、81.23%。说明电渗析脱盐方式较其他脱盐方式对于土鳖虫酶解液的损失小。同时脱盐率可以达到90%以上,同时考察酶解液的p H、固体浓度、脱盐时间对脱盐率的影响进行了考察,得出采用p H=6.5、生药浓度为500 mg/m L、脱盐时间在150 min时脱盐率可以达到90%以上。并采用方法学对电渗析脱盐工艺进行验证,得出方法可行、准确。结论:采用电渗析方法对土鳖虫酶解液进行脱盐,试验方法可行、准确、脱盐率高。
Objective ： Desalination of Eupolyphaga sinensis hydrolyzate was carried out in different ways, and the optimal desalination method and process were optimized. Methods： Desahing rate, peptide loss and solid matter retention were used as indexes, the methods of macroporous resin, mixed cation and anion resin, nanofiltration and electrodialysis were used to desalinate Eupolyphaga sinensis hydrolyzate, and the optimum process was optimized. Results：The desalting rates of the four methods were 95.19%, 60.97%, 90.01% and 94.43%, respectively, The rate of loss of peptide and the rate of solid matter retention were 32.12%,20.91%,40.55%, 10.12%;50.43%, 80.45%, 52.76% and 81.23%.The results showed that electrodialysis desahing method had less damage to the Eupolyphaga sinensis hydrolyzate than other desalting methods, and desalination rate can reach more than 90%. Meanwhile, on the desalination rate were investigated by the effects of pH, solid concentration and desahing time. The results showed that the desalting rate could reach over 90% when the pH value was 6.5, the crude drug concentration was 500 mg/mL and the desalting time was 150 min. The desalination process of electrodialysis was validated by the methodology which was feasible and accurate. Conclusion： Eleetrodialysis method is suitable for the desalting of Eupolyphaga sinensis hydrolyzate. The test method is feasible and accurate.
出处
《辽宁中医药大学学报》
CAS
2017年第8期46-49,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
"十二五"科技部重大新药创制专项项目(2013ZX09103002-021)
关键词
脱盐
土鳖虫酶解液
电渗析
desalination
Eupolyphaga sinensis hydrolyzate
electrodialysis
