摘要
目的:采用正交实验方法研究木瓜蛋白酶水解土鳖虫制备土鳖虫多肽的方法,并对土鳖虫多肽的体内免疫调节作用进行研究。方法:以水解度为指标,对酶解pH值、酶用量、温度计反应时间进行优化,获取最佳水解条件。通过观察小鼠免疫器官指数、碳粒廓清能力及血清白介素-2水平研究土鳖虫多肽对小鼠免疫功能的影响。结果:土鳖虫多肽的最佳酶解条件为:酶解pH值为8.0,酶用量为1%,反应温度为55℃,酶解时间为4.5 h。小鼠体内实验显示,与对照组相比,土鳖虫多肽可提高小鼠胸腺和脾脏指数,提高小鼠碳粒廓清能力及血清白介素-2的水平。结论:土鳖虫多肽具有免疫调节作用。
Objective ： Using papain to prepare polypeptide from Eupolyphaga sinensis, then study the immune function of polypep- tide from Eupolyphaga sinensis in vivo. Methods：Used hydrolysis degree as index, pH value, enzyme dosage, thermometer reaction time were optimized. Studied the influence of polyeptide on the mice immune functions through mice immune organs index,phagocytic func- tion and the level of IL-2. Results：The optimum enzymolysis condition was as follows： pH 8.0, enzyme 1%, temperature 55 ℃, reac- tion time 4. 5 h. In vivo test of mice demonstraed that, Eupolyphaga sinensis could elevate index of thymus and spleen, enhance the phagocytic function of macrophage and promote the level of IL-2 in serum. Conclusion： Eupolyphaga sinensis has immunoregulatory effect.
出处
《中药材》
CAS
CSCD
北大核心
2012年第9期1382-1385,共4页
Journal of Chinese Medicinal Materials
基金
江西省卫生厅科学基金(No.20092078)
江西省教育厅青年科学基金(No.GJJ10264)
关键词
土鳖虫
多肽
免疫调节
Eupolyphage sinensis Walker
Polypeptide
Immunoregulatory
