摘要
目的:了解不同热量限制方法对肥胖胰岛素抵抗大鼠能量代谢的影响。方法:将40只5周龄Wistar雄性大鼠随机分为两组继续饲养,其中空白组(n=5)以普通饲料喂养,另外35只采用高脂饲料喂饲,自由饮水,连续喂养8周。8周后肥胖胰岛素抵抗大鼠模型成功建立后,分为模型组、热量限制50%组(CR50%组)、间断禁食组(IF组)。热量限制50%组每日饲料供给量为模型组前一天平均进食量的50%。间断禁食组给予间断禁食的方法,1个周期包括2 d禁食(不禁水),5d自由进食,1个周期结束后重复下1个周期,共8个周期。其中空白组给予标准饲料,模型组、CR50%组、IF组给予继续给予高脂饲料。干预时间为8周。16周后下腔静脉取血约6 m L,检测FPG、FINS、Leptin、Ghrelin。结果:热量限制干预8周内,CR50%组和IF组的体质量较模型组下降(P<0.05),CR50%组的平均体质量比IF组更低(P<0.05)。模型组Lee’s指数较空白组明显升高(P<0.05),CR50%组及IF组Lee’s指数较模型组下降(P<0.05),CR50%组Lee’s指数比IF组下降(P<0.05)。模型组与空白组比较,内脏脂肪体质量比明显升高(P<0.05)。CR50%组、IF组与模型组比较,内脏脂肪体质量比下降(P<0.05)。CR50%组与IF组比较,差异无统计学意义(P>0.05)。模型组与IF组、CR50%组比较,Leptin水平上升,差异有统计学意义(P<0.05),Leptin水平在IF组和CR50%组无明显差异(P>0.05)。模型组与IF组、CR50%组比较,Ghrelin水平下降(P<0.05),IF组Ghrelin水平比CR50%组下降(P<0.05)。体质量与血Leptin成正相关(r=0.436,P=0.048),与Ghrelin负相关(r=-0.550,P=0.01)。内脏脂肪重量与血清Leptin水平正相关(r=0.402,P=0.04),与血清Ghrelin水平无相关性(P>0.05)。结论:(1)热量限制可以明显减轻大鼠体质量、内脏质量。比较IF组,CR50%具有较好的减重效应。(2)热量限制可以改善瘦素抵抗。
Objective： To investigate the effects of different caloric restriction （CR） on the energy metabolism of obese insulin resistant rats. Methods： （1） obese insulin resistant rat model： 40 Wistar rats were randomly divided into control group （n=5）, obese insulin resistant group （n=35）. Control group rats were fed with standard diet, and obese insulin resistant group rats were fed HFD （high fat diet） for 8 weeks. At the end of 8 weeks, fasting plasma glucose （FPG）, fasting insulin （FINS） was tested, HOMA-IR of obese insulin resistant group significantly increased compared with control group, and then chose rats heavier than average weight of control group by 20% as the obese IR rats. （2） Different CR treatments： obese insulin resistant rats were randomly divided into three groups; obese insulin resistant rats without any treatment, CR 50% group and IF group （Intermittent fasting）. Diet supply for CR50% group was one half of obese insulin resistant rats＂ daily supply. IF group was fed with no food but water for 2 days, and ad libitum for 5 days followed the cycle by 8 times, These three groups were kept with HFD. The treatments continued for 8 weeks. （3） Collecting samples： at the end of 16 weeks, rats were sacrificed, blood samples were collected, visceral adipose tissue were cut.Plasma glucose, serum insulin, serum Leptin and serum Ghrelin in blood were measured. Results： Body weight, Lee＇s index, ratio of visceral fat to body weight： after 8 weeks of CR, BWs （body weight） of control group and IF group were lower than that of obese insulin resistant group （P〈0.05）. Compared to that of IF group, BW of CR50% was lower （P〈0.05）. Lee＇s index of obese insulin resistant group was dramatically higher than that of CRS0% group and IF group （P〈0.05）. Lee＇s index of CR group was lower than that of IF group （P〈0.05）. When compared with obese insulin resistant group, ratios of visceral fat to body weight （%） in CR50% group and IF group w
出处
《中医药导报》
2017年第16期26-32,共7页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
广东省自然科学基金﹙博士启动基金﹚:<基于Wnt/β-catenin/PPARγ信号通路探讨健脾化痰方对代谢综合征脂肪细胞分化的作用机制>(2014A030310249)
广东省中医药管理局课题:<健脾化痰方对代谢综合征大鼠糖脂代谢及于Wnt/β-catenin/PPARγ信号通路影响的研究>(20151160)
关键词
热量限制
肥胖
胰岛素抵抗
大鼠
摄食
能量代谢
caloric restriction
obesity
insulin resistance
rat
food intake
energy metabolism
