摘要
目的:探讨内质网应激IRE1通路在染氟雄性大鼠生殖系统中的作用。方法:60只4周龄SD雄性大鼠随机分为4组,包括对照组、Na F组、NAC组和Na F+NAC组,每组15只,采用等体积经口灌胃方式处理,每周灌胃6 d停灌1 d,连续灌胃7周。然后测定睾丸脏器系数,酶联免疫法测定血清中睾酮(T)、卵泡刺激素(FSH)、促黄体生成素(LH)含量,TUNEL法检测睾丸细胞凋亡情况,qRT-PCR法检测大鼠睾丸组织中IRE1及JNK mRNA的相对表达量,Western blot法检测大鼠睾丸组织中IRE1、p-IRE1、JNK和p-JNK蛋白的相对表达量。结果:与对照组相比,Na F组体重及睾丸质量减轻,血清T含量降低,血清FSH、LH含量升高,大鼠睾丸细胞凋亡指数升高,大鼠睾丸组织中IRE1及JNK mRNA表达水平均升高,p-IRE1及p-JNK蛋白表达水平均升高(P均<0.05)。与Na F组比较,Na F+NAC组大鼠睾丸细胞凋亡指数降低,大鼠睾丸组织中IRE1及JNK mRNA表达水平均下降,p-JNK蛋白表达水平下降(P均<0.05)。结论:氟暴露可以诱发内质网应激IRE1通路介导的JNK凋亡信号通路;NAC抑制内质网应激,对氟致雄性大鼠生殖损伤起到了一定的拮抗作用。
Aim：To research the role of IRE1 endoplasmic reticulum stress pathway in the fluoride-exposed male rat reproductive system injury.Methods： A total of sixty healthy male SD rats（4 weeks old） were divided into 4 groups randomly,including control group,NAC group,NaF group and NaF＋NAC group.Each group had 15 rats.The rats were administered for 7 weeks,during which the rats were continuously treated by gavage for 6 days per week.Weight coefficient of testises was assayed.Testosterone（T）,follicle stimulating hormone（FSH） and luteinizing hormone（LH） were determined by enzyme-linked immunosorbent assay.TUNEL method was used to detect testis cell apoptosis.IRE1 and JNK mRNA expression levels were detected by qRT-PCR.IRE1,p-IRE1,JNK and p-JNK protein expression levels were assayed by Western blot.Results： Compared with control group,in NaF group body weight and testicular weight were decreased,serum T level was decreased,serum FSH and LH levels were elevated,testicular apoptosis index was increased,the expression levels of IRE1 and JNK mRNA were increased,and protein expression levels of p-IRE1 and p-JNK were also increased significantly（P〈0.05）.Compared with NaF group,in NaF＋NAC group the apoptosis index was reduced,the expression levels of IRE1 and JNK mRNA were down-regulated,and protein xpression level of p-JNK was decreased significantly（P〈0.05）.Conclusion： Fluorine exposure could induce endoplasmic reticulum stress,and activate IRE1 mediated JNK apoptosis signaling pathway.NAC could inhibit endoplasmic reticulum stress,playing a protective role in fluoride-exposed male rat reproductive system injury.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2017年第4期403-407,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省教育厅科学技术研究重点项目13A330735
关键词
氟
内质网应激
IRE1通路
生殖系统
大鼠
fluorine
endoplasmic reticulum stress
IRE1 pathway
reproductive system
rat
