摘要
目的:研究地龙组分对单侧输尿管结扎大鼠BMP-7、Smad6、TGF-β_1表达的影响,探讨地龙组分延缓肾纤维化的可能机制。方法:健康雄性wistar大鼠60只,随机分组:假手术组+模型组+地龙低剂量组+地龙高剂量组。采用单侧输尿管结扎法复制肾间质纤维化大鼠模型,取梗阻侧肾组织标本,免疫组织化学方法检测肾组织BMP-7、Smad6、TGF-β_1的表达。结果:地龙治疗组大鼠的肾间质纤维化有所改善;与模型组相比,地龙治疗组大鼠肾组织BMP-7、Smad6表达增多,TGF-β_1表达下降(P<0.05)。结论:地龙组分通过上调BMP-7、Smad6表达,下调TGF-β_1的表达,来延缓肾纤维化的进程。
Objective： To investigate the effete of Di Long compostion on the expression of bone motphogenetic protein-7（ BMP-7） and Transforming growth factor-β_1（ TGF-β_1） and Smad6 in renal tissue of rats by unilateral ureteral obstruetion（ UUO）,and to illustrate the mechanism of Di Long compostion on delaying renal interstitial fibrosis. Methods： In the study,60 male wistar rats were randomly divided into groups in cluding sham-operated group,model group,low-dose group of Di Long Capsule,high-dose group of Di Long Capsule. the rats by unilateral ureteral obstruetion（ UUO） was adopted. The expression of BMP-7、Smad6 and TGF-β_1 in renal tissue were determined by immuno histoehemistry with two steps. Results： renal interstitial fibrosis of the rats treated by Di Long compostion was improved,the expression of TGF-β_1decreased and the expression of BMP-7 and Smad6 increased significantly compared with model group. Conclusion： Di Long compostion can decrease the expression of TGF-β_1and increase the expression of BMP-7 and Smad6 in renal tissue of UUO rats,it is possible mechanism of improving renal interstitial fibrosis.
出处
《中国中西医结合肾病杂志》
2017年第6期486-489,I0001,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
黑龙江省自然科学基金资助项目(No.H2019059)
