摘要
为了分离筛选耐高温的纤维素高效降解菌,从鸡粪蘑菇渣高温堆肥中取样,采用滤纸富集培养基进行富集培养、刚果红纤维素培养基进行分离纯化培养、滤纸条培养基和刚果红纤维素培养基进行初筛培养、液体发酵测定纤维素酶活法进行复筛培养,利用形态特征观察、生理生化特征检测和基于16SrRNA基因序列的系统发育分析法进行初步鉴定,结果分离筛选到1株耐高温纤维素降解菌XD-3.该菌株在50℃生长良好、羧甲基纤维素酶(CMCase)活力达6.14±0.37U/mL.综合形态特征、生理生化特性、基于16S rRNA基因序列的系统发育分析,菌株XD-3初步鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens).
In order to obtain high-temperature resistant cellulose-degrading bacteria, samples were collected from chicken manure and mushroom residue compost. After being enriched through filter paper medium, high-temperature resistant cellulose-degrading bacteria were isolated and purified on cellulose Congo red medium. The isolates were preliminarily screened through filter medium and cellulose Congo red medium,and then were further screened through quantitative analysis of cellulase activity of liquid fermen- tation broth. A high-temperature resistant cellulose-degrading bacterium, designated strain XD-3,was obtained,which grew well at 50 ℃ and the carboxymethyl-cellulase （CMCase） activity was 6.14±0.37 U/ mL. The combination of morphology, physiological/biochemical characterization and phylogenetic analysis based on 16S rRNA gene sequence showed that strain XD-3 was a member of a known Bacillus species, Bacillus am yloliquefaciens.
出处
《吉首大学学报（自然科学版）》
CAS
2017年第4期72-77,共6页
Journal of Jishou University(Natural Sciences Edition)
基金
国家自然科学基金资助项目(31460004)
湖南省重点学科建设项目(JSU071312Z01)
吉首大学校级科研项目(JDY16010)
关键词
耐高温纤维素降解菌
16S
RRNA基因
系统发育分析
解淀粉芽孢杆菌
high-temperature resistant cellulose-degrading bacteri
16S rRNA gene
phylogenetic analysis
Bacillus am yloliquefaciens
