摘要
以瘤背石磺为原料,采用响应面法优化瘤背石磺活性多肽的制备工艺,考察Na Cl浓度、液固比、温度和提取时间对羟基自由基清除率的影响。结果表明:最优提取条件为Na Cl浓度0.09%,液固比57 m L/g,提取时间2.5 h,在此条件下羟基自由基清除率最大,预测值为33.09%,实测值为33.34%,实测值与预测值相接近,且相对误差只有0.76%,表明响应面法得到的最佳提取条件合理可行。天然活性多肽体外抗氧化活性实验结果表明:其对羟基自由基、DPPH自由基、H2O2有较好清除能力,还具有一定还原能力以及亚铁离子螯合能力。总体而言,瘤背石磺天然活性多肽表现出一定的体外抗氧化活性,具有良好的开发前景。
In this paper, the preparation process of oyster bioactive peptides was optimized by response surface method. The effect of NaC1 concentration,liquid-solid ratio, temperature and extraction time on the scavenging rate of hydroxyl radicals was investigated.The result showed that optimum extraction conditions were：NaC1 concentration 0.09% ,liquid-solid rate 57 mE/g, time 2.5 h.Under this condition, the predictive value clearance rate of hydroxyl radical was 33.09% and the measured value was 33.34%, and the relative error was only 0.76% .It showed that the optimum extracting condition of response surface method was reasonable and feasible. The best result of bioactive peptides antioxidant activity indicated that it had a relatively good scavenging ability to hydroxyl radical, DPPH free radical and H20a.Meanwhile, it also had reducing power and ferrous ion chelating capacity.On the whole, Onchidium struma bioactive peptides reflect a certain antioxidant activity and has a good prospect of development.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第17期168-173,共6页
Science and Technology of Food Industry
基金
国家自然科学基金项目(41276157)
上海高校水产学一流学科建设项目资助
关键词
瘤背石磺
响应面法
活性多肽
抗氧化性
Onchldium struma
response surface methodology
bioactive peptides
antioxidant activity
