摘要
目的:研究黄根单体(HG2、HG3、HG4)对大鼠肝星状细胞(HSC-T6)增殖的抑制作用以及诱导其凋亡的影响。方法:采用四甲噻唑蓝(MTT)法检测黄根单体对HSC-T6的增殖的抑制作用,并运用流式细胞术检测细胞凋亡率。结果:单体HG3可诱导HSC-6发生凋亡,对其增殖具有抑制作用,且具有剂量依赖性;而单体HG2和单体HG4对大鼠HSC-6表现出较弱的抑制和诱导凋亡作用。结论:黄根的蒽醌类成分能抑制HSC-T6细胞增殖并诱导其发生凋亡,从而发挥抗肝纤维化作用。
Objective：To investigate the effects of Prismatomeris connata Y. Z. Ruan. Monomers on Rat Hepatic Stellate Cell （ HSC- T6）. Methods： MTr assay was used to evaluate the inhibitory effect of the drugs on HSC-T6 , flow cytometry were applied to measure the cell apoptosis. Results： HG3 not only could inhibit proliferation of HSC-T6 cells in concentration manners, but also could induce apoptosis of HSC-T6 cells. HG2 and HG4 showed a slight effect on the Proliferation apoptosis in Rat Hepatic Stellate Cell. Conclusion： The Prismatomer- is connata Y. Z. Ruan. Monomers can inhibit the proliferation of HSC-T6 cells and induce apoptosis, wihch show the therapeutical effects on hepatic fibrosis.
出处
《中西医结合肝病杂志》
CAS
2017年第4期228-230,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
国家自然科学基金课题(No.81260683)
关键词
黄根
单体
肝星状细胞
增殖
抑制
凋亡
Prismatomeris connata Y. Z. Ruan.
Monomers
hepatic stellate cells
Proliferation
inhibition
Apoptosis
