摘要
考察隔山消提取物中4个活性成分青阳参苷元、去酰基萝藦苷元、白首乌二苯酮和东莨菪内酯在正常与功能性消化不良(FD)模型大鼠尿液、粪便中的排泄情况。大鼠分为正常组和FD模型组,模型组采用灌胃冰盐酸结合不规则饲养诱导FD模型。按1 g·kg^(-1)的剂量分别灌胃隔山消提取物,分别在4、8、12、24、36、48、60、72、84 h收集大鼠尿液和粪便进行UPLC-ESI-MS/MS分析,比较4个成分在正常和FD大鼠中的排泄情况。结果表明,除粪便中的白首乌二苯酮外,84 h内青阳参苷元等成分在尿液和粪便中均未达到坪值。青阳参苷元主要经粪便排泄,正常和FD模型状态下的累积排泄率分别为0.32%、0.66%;去酰基萝藦苷元主要经尿液排泄,正常和FD模型状态下的累积排泄率分别为6.70%、7.56%;白首乌二苯酮正常状态下主要经粪便排出,FD模型状态下主要经尿液排泄,累积排泄率分别为0.41%、0.52%;东莨菪内酯主要经尿液排泄,正常和FD模型状态下的累积排泄率分别为0.83%、2.13%。总体来看,FD状态下各成分主要经尿液排出,与正常组相比,FD组中青阳参苷元、白首乌二苯酮、东莨菪内酯在尿液中的累积排泄率显著降低(P<0.05)。可见FD状态对隔山消主要成分的排泄有一定影响,同时各成分经尿液、粪便的排泄量均较低,提示其经口服给药后可能存在较为广泛的代谢途径,主要以代谢产物的形式排出体外。该实验为隔山消的新药开发和临床合理应用提供参考依据。
In the present study,the excretion of four active components(qingyangshengenin,deacylmetaplexigenin,baishouwu benzophenone,and scopoletin)in Cynanchum auriculatum extract in the urine and feces of normal and functional dyspepsia(FD)rats was investigated.Rats were divided into a normal group and an FD model group.The FD model was induced by oral administration of ice hydrochloric acid combined with irregular feeding.The C.auriculatum extract was administered orally at a dose of 1 g·kg^(-1).The rat urine and feces were collected at 4,8,12,24,36,48,60,72,and 84 h for UPLC-ESI-MS/MS analysis.The differences in excretion of the four components were compared between normal and FD rats.The results showed that except for the baishouwu benzophenone in the feces,the components such as qingyangshengenin in the urine and feces did not reach the plateau value within 84 h.Qingyangshengenin was mainly excreted through defecation,and the cumulative excretion rates in the normal state and FD were 0.32%and 0.66%,respectively.Deacylmetaplexigenin was mainly excreted through urination,and the cumulative excretion rates in the normal state and FD were 6.70%and 7.56%,respectively.Baishouwu benzophenone was mainly excreted through defecation in the normal state,but mainly excreted through urination in the FD state,with cumulative excretion rates of 0.41%and 0.52%,respectively.Scopoletin was mainly excreted through urination,with cumulative excretion rates of 0.83%and 2.13%in the normal state and FD,respectively.In general,the components were mainly excreted in the urine in the FD state.Compared with the normal group,the FD group showed decreased cumulative excretion rates of qingyangshengenin,baishouwu benzophenone,and scopoletin in the urine(P<0.05).Therefore,FD had a certain influence on the excretion of the main components of C.auriculatum extract,and the excretion of each component through urination and defecation was low,suggesting that there might be a wide range of metabolic pathways after oral administration and components wer
作者
孙佳
勾健
刘利琴
刘春花
潘洁
李月婷
巩仔鹏
SUN Jia;GOU Jian;LIU Li-qin;LIU Chun-hua;PAN Jie;LI Yue-ting;CONG Zi-peng(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,China;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine(Ministry of Education),Guizhou Medical University,Guiyang 550004,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550004,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第23期6355-6364,共10页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81760700)
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2021-087)
贵州医科大学博士启动基金项目(校博合J字[2020]013号)。
关键词
隔山消
排泄
尿液
粪便
功能性消化不良
Cynanchum auriculatum
excretion
urine
feces
functional dyspepsia
