摘要
目的探讨外源性ghrelin对糖尿病心肌病(DCM)大鼠心肌动作电位及瞬时外向钾电流(Ito)的影响。方法雄性SD大鼠腹腔注射链脲佐菌素(60mg/kg)诱导Ⅰ型DCM模型,并采用全细胞膜片钳技术观察ghrelin对糖尿病大鼠心室肌细胞动作电位及瞬时外向钾电流的影响。结果与DCM组比较,10^(-7) mmol/L ghrelin明显延长DCM大鼠心室肌细胞5%动作电位时程(APD50)[(12.49±2.32)%;n=7,P=0.037]及APD90[(26.29±5.13)%;n=7,P=0.006];并明显抑制Ito电流峰值,抑制率[(23.14±3.07)%;n=9,P=0.021]。结论外源性ghrelin抑制Ito电流而延长DCM大鼠心肌动作电位时程,参与糖尿病大鼠心脏电生理的重构。
Objective To study the effects of ghrelin on action potential （AP） and transient outside K^＋ current （I,o） in ventricular myocytes of diabetic cardiomyopathy （DCM） rats. Methods A rat model of DCM was established by single intraperitoneal injection of streptozotocin （STZ, 60 mg/kg）, and whole cell patch-clamp technique was used to record the effects of 10.7 mmol/L ghrelin on AP and /to current density in the DCM rat ventricular myocytes. Results Compared to DCM group, Ghrelin of 10.7 mmol/L could significantly prolong APDs0[（12.49±2.32）%; n=7, P=0.037] and APD90[（26.29±5.13）%; n=7, P=0.006] and decrease /to current peak value [（23. 14± 3.07）% ; n = 9, P= 0. 021] in DCM rat ventricular myocytes. Conclusion Exogenous ghrelin is involved in the electrophysiological reconstruction of the heart of diabetic rats by decreasing /to current and prolonging APD in STZ-induced DCM rat ventricular myocytes.
作者
冯丹阳
孙强
陈莉娜
赵正杭
周筠
FENG Da n-yang SUN Qiang CHEN Li-na ZHAO Zheng-hang ZHOU Jun(Department of Pharmacology, School of Basic Medical Sciences of Xi＇an Jiaotong University Health Science Center, Xi＇an 710061 Department of Pharmacy, Xi＇an Electric Central Hospital, Xi＇an 710032, China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2017年第5期661-664,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金面上项目(No.81170176)
陕西省科技统筹创新工程计划项目(No.2014KTCL03-10)~~
