摘要
以椰糠作为碳源,从红树林土壤中分离了一株高产纤维素酶的真菌,命名为DZ10。经形态、生理生化和分子生物学试验,鉴定菌株DZ10为长枝木霉菌(Trichoderma longibrachiatum)。该菌在pH值为6.5、培养温度为35℃、初始NaCl含量为2.0%、添加K^+终浓度为0.1 mmol/L条件下,椰糠降解率最高为39.88%;在pH值为6.5、培养温度40℃、初始NaCl含量3.0%、添加K^+终浓度为0.1 mmol/L条件下,羧甲基纤维素酶活力(CMCA)最高值为80.07 U/mL;在pH值为6.0、培养温度35℃、初始NaCl含量1.5%、添加K^+终浓度为0.1 mmol/L条件下,滤纸酶活力(FPA)最高值为73.81 U/mL。Ca^(2+)、K+对菌株DZ10产酶和椰糠降解率有促进作用,Mg^(2+)抑制菌株DZ10产酶和椰糠降解率。
Using the coconut coir as a carbon source, a high cellulase-producing strain named DZ10 was isolated from mangrove soils. By morphology identification, physiological and biochemical properties and molecular biological experiments, strain DZ10 was identified as Trichoderma longibrachiatum. Under the conditions of pH 6.5, culture temperature 35 ℃, initial NaCl concentration 2.0% and K＋ final concentration 0.1 mmol/L, the coconut coir degradation rate was the highest of 39.88%. Under the conditions of pH 6.5, culture temperature 40 ℃, initial NaCl concentration 3.0% and K^＋ final concentration 0.1 mmol/L, the activity of carboxymethyl cellulase （CMCA） was the highest of 80.07 U/ml. Under the conditions of pH 6.0, culture temperature 35 ℃, initial NaCl concentration 1.5% and K＋ final concentration 0.1 mmol/L, the activity of filter paper lyase was the highest of 73.81 U/ml. Ca^2＋ and K^＋ promoted the enzyme production and the degradation rate of strain DZ10, but Mg^2＋ inhibited the enzyme production and the degradation rate of strain DZ10.
作者
雷湘兰
沈振国
孙倩
郑金明
LEI Xianglan SHEN Zhenguo SUN Qian ZHENG Jinming(Department of Tropical Agricultural Technology, Hainan College of Vocation and Technique, Haikou 570216, China)
出处
《中国酿造》
CAS
北大核心
2017年第8期72-75,共4页
China Brewing
基金
海南省自然科学基金项目(314094)
海南省教育厅科研基金项目(HNJG2014-85)
关键词
椰糠
纤维素酶
分离
鉴定
降解
coconut coir
cellulase
isolation
identification
degradation
