摘要
目的选取总黄酮最优提取工艺,为芒果叶的进一步开发研究提供科学依据。方法以百色芒果叶为原料,分别采用普通醇提法和超声提取法,进行单因素试验和正交试验,分析乙醇浓度、液固比、提取时间和提取温度对总黄酮提取效果的影响,并以总黄酮得率为评价指标,优化提取工艺。结果乙醇加热回流法的最佳工艺为温度100℃,时间2 h,液固比10,提取次数3次;超声提取法的最佳工艺为温度50℃,时间35 min,液固比10,提取次数3次。结论芒果叶中总黄酮的最佳提取工艺为乙醇加热回流法。
Objective To optimize the extraction process of total flavonoids,and provide a scientific basis for further development and utilization of Mangifera Indica leaves.Methods Baise Mangifera Indica leaves as raw materials,the common ethanol extraction and ultrasonic extraction method were used,single factor test and orthogonal test were used,the effects of alcohol concentration,liquid-solid ratio,extraction temperature and extraction time on the extraction of total flavonoids were analyzed,and the yield of total flavonoids as index,the extraction process optimization.Results The optimum ethanol refluxing method for temperature was 100 ℃,the time was 2 h,the ratio of liquid to solid was 10,the times of extraction was 3.The optimum ultrasonic extraction method for temperature was 50 ℃,the time was 35 min,the ratio of liquid to solid was 10,the times of extraction was 3.Conclusion The best extraction process of total flavonoids from Mangifera Indica leaves is ethanol refluxing method.
作者
张帆
陈越
侯文浩
邓国文
刘小幸
周程艳
Zhang Fan Chen Yue Hou Wenhao Deng Guowen Liu Xiaoxing Zhou Chengyan(Department of Pharmacy, Hebei University, Baoding, Hebei, China 071000)
出处
《中国药业》
CAS
2017年第15期5-10,共6页
China Pharmaceuticals
基金
2016年"挑战杯"河北大学课外学术科技作品竞赛项目[ZR038]
2016年河北大学大学生创新创业训练计划项目[2016182]
关键词
总黄酮
单因素试验
正交试验
提取工艺
total flavonoids
single factor experiment
orthogonal test
extraction technology
