摘要
目的:观察活血通督汤对脊髓损伤后肢体运动功能和胶质瘢痕形成的影响,探讨活血通督汤治疗脊髓损伤的作用机制。方法:成年SD大鼠18只,采用随机数表法随机分为假手术组、模型组和活血通督汤组,每组各6只。假手术组仅行椎板切除术,模型组和活血通督汤组采用NYU脊髓打击器建立脊髓损伤模型,造模后假手术组和模型组给予生理盐水灌胃,活血通督汤组给予活血通督汤灌胃。术后第1,3,5,7天行BBB肢体运动功能评分,7d后取材。采用尼氏染色法观察神经元细胞形态结构以及神经元受损情况,免疫荧光检测胶质纤维酸性蛋白(GFAP)表达,计算GFAP阳性细胞数。结果:自术后第3天起,活血通督汤组的大鼠BBB评分高于模型组大鼠BBB评分,差异有统计学意义(P<0.05);尼氏染色结果显示活血通督汤组神经元受损情况得到改善,神经元的形态结构优于模型组;免疫荧光检测结构显示与假手术组相比,脊髓损伤后GFAP表达显著,损伤处GFAP阳性细胞数增多,差异有统计学意义(P<0.05);与模型组相比,活血通督汤组GFAP阳性细胞数较少,差异有统计学意义(P<0.05)。结论:活血通督汤能促进脊髓损伤后肢体运动功能的恢复,其机制可能与抑制GFAP表达、降低脊髓损伤后胶质瘢痕形成有关。
Objective：To discuss the effects of the recovery of locomotion in rats via Huoxue Tongdu decoction,the morphology and structure of neurons,the damage of neuron and the expression of glial fibrillary acidic protein（GFAP）.Methods：18 adult SD rats were randomized into three groups：the sham operation group,the model group and the Huoxue Tongdu decoction group with six rats in each group.The sham operation group was undergoned laminectomy while the model group and Huoxue Tongdu decoction group with NYU spinal cord impactor to establish the spinal cord injury model.The sham operation group and model group were given saline while the Huoxue Tongdu decoction group the same amount of Huoxue Tongdu decoction perday after operation.BBB score was used to evaluate the locomotion daily.After 7d,tissues were gathered for Nissl＇s staining and immunofluorescence.Results：There is significanty difference between Huoxue Tongdu decoction group than the other two groups in the BBB score from the third day on（P〈0.05）.A better structure and morphology of neuron was observed by Nissl＇s staining in the Huoxue Tongdu decoction group compared with the model group which suggests that Huoxue Tongdu decoction reduced GFAP secretion from cells of the spinal cord（P〈0.05）.Conclusions：It suggests that Huoxue Tongdu decoction can alleviate locomotion function after spinal cord injury by inhibiting the expression of GFAP positive cells and promoting the regeneration of injured axons
作者
汪今朝
范筱
张俐
WANG Jinzhao FAN Xiao ZHANG Li(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China Xiamen Medical School, Xiamen 361008, Fuj ian China.)
出处
《中国中医骨伤科杂志》
CAS
2017年第8期1-5,共5页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金项目(81273775)
福建省百千万领军人才项目
关键词
脊髓损伤
活血通督汤
胶质纤维酸性蛋白
胶质瘢痕
spinal cord injury
Huoxue Tongdu decoction
glial fibrillary acidic protein（GFAP）
glial scar
